Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.

A physical, genetic and functional sequence assembly of the barley genome.

CORDERO, Francesca;BECCUTI, Marco;
2012-01-01

Abstract

Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
2012
491
711
716
Genomics; Plant sciences; Genetics
Mayer KF; Waugh R; Langridge P; Close TJ; Wise RP; Graner A; Matsumoto T; Sato K; Schulman A; Muehlbauer GJ; Stein N; Ariyadasa R; Schulte D; Poursarebani N; Zhou R; Steuernagel B; Mascher M; Scholz U; Shi B; Langridge P; Madishetty K; Svensson JT; Bhat P; Moscou M; Resnik J; Close TJ; Muehlbauer GJ; Hedley P; Liu H; Morris J; Waugh R; Frenkel Z; Korol A; Bergès H; Graner A; Stein N; Steuernagel B; Scholz U; Taudien S; Felder M; Groth M; Platzer M; Stein N; Steuernagel B; Scholz U; Himmelbach A; Taudien S; Felder M; Platzer M; Lonardi S; Duma D; Alpert M; Cordero F; Beccuti M; Ciardo G; Ma Y; Wanamaker S; Close TJ; Stein N; Cattonaro F; Vendramin V; Scalabrin S; Radovic S; Wing R; Schulte D; Steuernagel B; Morgante M; Stein N; Waugh R; Nussbaumer T; Gundlach H; Martis M; Ariyadasa R; Poursarebani N; Steuernagel B; Scholz U; Wise RP; Poland J; Stein N; Mayer KF; Spannagl M; Pfeifer M; Gundlach H; Mayer KF; Gundlach H; Moisy C; Tanskanen J; Scalabrin S; Zuccolo A; Vendramin V; Morgante M; Mayer KF; Schulman A; Pfeifer M; Spannagl M; Hedley P; Morris J; Russell J; Druka A; Marshall D; Bayer M; Swarbreck D; Sampath D; Ayling S; Febrer M; Caccamo M; Matsumoto T; Tanaka T; Sato K; Wise RP; Close TJ; Wannamaker S; Muehlbauer GJ; Stein N; Mayer KF; Waugh R; Steuernagel B; Schmutzer T; Mascher M; Scholz U; Taudien S; Platzer M; Sato K; Marshall D; Bayer M; Waugh R; Stein N; Mayer KF; Waugh R; Brown JW; Schulman A; Langridge P; Platzer M; Fincher GB; Muehlbauer GJ; Sato K; Close TJ; Wise RP; Stein N.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/125624
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