nanobiotechnology. Among the various preparation methods, polymerization in microemulsion of reverse micelles results in spherical NPs highly homogeneous in size, with relevant advantages in behaviour homogeneity in both cell-free and cell tests and applications. Most part of the performances of such NPs in biological media are ruled by their surface/interface features, that have been the subject of this work. The converging use of different methodologies (HR-TEM, Z-potential, DLS, FT-IR, BET, and TGA) made allowance to establish that NPs (50±2 nmin size; monodispersed in water) exhibit a structured, highly hydroxylated surface, where all silanols are engaged in H-bonding with neighbour –OH. Such a rich hydroxyl layer appeared poorly active towards protein (BSA) adsorption (ca. 50% of the theoretical side-on monolayer), producing a thin, incomplete corona around the silica core. Moreover, by in situ FT-IR ATR and UV-CD, it was found that adsorbed BSA underwent severe modifications in terms of both tertiary and secondary structure.

Surface features and interfacial behaviour towards proteins of engineered silica nanoparticels

CATALANO, FEDERICO;ALBERTO, Gabriele;COLUCCIA, Salvatore;MARTRA, Gianmario
2011-01-01

Abstract

nanobiotechnology. Among the various preparation methods, polymerization in microemulsion of reverse micelles results in spherical NPs highly homogeneous in size, with relevant advantages in behaviour homogeneity in both cell-free and cell tests and applications. Most part of the performances of such NPs in biological media are ruled by their surface/interface features, that have been the subject of this work. The converging use of different methodologies (HR-TEM, Z-potential, DLS, FT-IR, BET, and TGA) made allowance to establish that NPs (50±2 nmin size; monodispersed in water) exhibit a structured, highly hydroxylated surface, where all silanols are engaged in H-bonding with neighbour –OH. Such a rich hydroxyl layer appeared poorly active towards protein (BSA) adsorption (ca. 50% of the theoretical side-on monolayer), producing a thin, incomplete corona around the silica core. Moreover, by in situ FT-IR ATR and UV-CD, it was found that adsorbed BSA underwent severe modifications in terms of both tertiary and secondary structure.
2011
European Biotechnology Congress 2011
Istanbul
28/09-01/10 2011
22
S119
S119
nanoparticles; interface; protein adsoprtion
F. Catalano; G. Alberto; S. Coluccia; G. Martra
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/131216
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