The peripheral nervous system maintains a high ability to regenerate in adult life. Moreover after nerve injury with high tissue defect a complete functional recovery is not possible and a surgical operation is requested to promote the regeneration. Among the advanced strategies to promote nerve repair artificial conduits are the most promising, but is still an open question which manipulation of the internal lumen gives the best results in regeneration outcome. One approach is filling the tube with biomaterials enriched with neurotrophic factors in order to stimulate and accelerate regeneration processes. In the present study we tested in vitro a combination of a semi-solid gel (composed mainly by hyaluronic acid and laminin) and iron oxide-conjugated growth factors, proposed as a possible delivery system for nerve conduits engineering. We focused on three neuronal growth factors: nerve growth factor (NGF), glial cell line derived growth factor (GDNF) and basic fibroblast growth factor (FGF-2), all factors well known for their ability to promote neuronal survival and growth. Using dorsal root ganglion (DRG) model we evaluated the biological activity of conjugated factors and their ability to promote neurite outgrowth when administrated with cell medium or mixed in a gel. Our preliminary study showed that conjugated factors are able to promote neurite outgrowth as free factors, also in case of DRG culturing in a gel layer enriched with factors. Further experiments are still ongoing to asses the beneficial effect of using these conjugated factors combined to gel as a microenvironment favourable for nerve regeneration.

IN VITRO ANALYSIS ON NVR-GEL COMBINED WITH IRON OXIDE-CONJUGATED FACTORS FOR PERIPHERAL NERVE ENGINEERING

MORANO, MICHELA;FREGNAN, Federica;GEUNA, Stefano
2014-01-01

Abstract

The peripheral nervous system maintains a high ability to regenerate in adult life. Moreover after nerve injury with high tissue defect a complete functional recovery is not possible and a surgical operation is requested to promote the regeneration. Among the advanced strategies to promote nerve repair artificial conduits are the most promising, but is still an open question which manipulation of the internal lumen gives the best results in regeneration outcome. One approach is filling the tube with biomaterials enriched with neurotrophic factors in order to stimulate and accelerate regeneration processes. In the present study we tested in vitro a combination of a semi-solid gel (composed mainly by hyaluronic acid and laminin) and iron oxide-conjugated growth factors, proposed as a possible delivery system for nerve conduits engineering. We focused on three neuronal growth factors: nerve growth factor (NGF), glial cell line derived growth factor (GDNF) and basic fibroblast growth factor (FGF-2), all factors well known for their ability to promote neuronal survival and growth. Using dorsal root ganglion (DRG) model we evaluated the biological activity of conjugated factors and their ability to promote neurite outgrowth when administrated with cell medium or mixed in a gel. Our preliminary study showed that conjugated factors are able to promote neurite outgrowth as free factors, also in case of DRG culturing in a gel layer enriched with factors. Further experiments are still ongoing to asses the beneficial effect of using these conjugated factors combined to gel as a microenvironment favourable for nerve regeneration.
2014
FENS Forum of Neuroscience
Milano
5-9 luglio 2014
-
-
-
peripheral nerve regeneration; iron oxide nanoparticle
M. Morano; F. Fregnan; M. Wesemann; O. Ziv-Polat; A. Shahar; C. Grothe; K. Haastert-Talini; S.Geuna
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/148771
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact