In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and antiinflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd- HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.

Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

GENINATTI CRICH, Simonetta
First
;
LANZARDO, Stefania;CONTI, Laura;RUIU, ROBERTO;ALBERTI, DIEGO;CAVALLO, Federica;CUTRIN, Juan Carlos
Co-last
;
AIME, Silvio
2015-01-01

Abstract

In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and antiinflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd- HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.
2015
7
15
6527
6533
http://www.rsc.org/publishing/journals/NR/Index.asp
Materials Science (all)
Geninatti Crich, S; Cadenazzi, M.; Lanzardo, S.; Conti, L.; Ruiu, R.; Alberti, D.; Cavallo, F.; Cutrin, J.C.; Aime, S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1524269
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