There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β-glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera

A murine, bispecific monoclonal antibody simultaneously recognizing β-Glucan and MP65 determinants in Candida species

ZITO, ANDREA;MANDILI, GIORGIA;HORENSTEIN, ALBERTO;MALAVASI, Fabio;
2016-01-01

Abstract

There is a real medical need of new diagnostic tools for the early recognition of invasive Candida infections. We exploited a rather simple and rapid redox methodology to construct a bispecific monoclonal antibody (bsmAb) that combines a monoclonal antibody (mAb) directed against 1,3-β-D-glucan, a well-known, pan-fungal diagnostic biomarker, with a mAb recognizing MP65, a major immunogenic mannoprotein secreted by C.albicans and other Candida species. The bsmAb (MP65/bglu mAb) was successfully produced and purified at high yields and proved to bind and reveal simultaneously, with high sensitivity, the β-glucan and MP65 antigens in both purified and native forms. The MP65/bglu mAb is the first bispecific antibody generated against a fungal microorganism and may prove useful for the concurrent detection of different and clinically significant Candida biomarkers in patient sera
2016
11
2
e0148714
e0148714
http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0148714&representation=PDF
Animals; Antibodies, Bispecific; Antibodies, Fungal; Antibodies, Monoclonal; Antibody Specificity; Antigens, Fungal; Biomarkers; Candida; Candida albicans; Candidiasis, Invasive; Fungal Proteins; Humans; Immunodominant Epitopes; Membrane Glycoproteins; Mice; Serologic Tests; beta-Glucans; Agricultural and Biological Sciences (all); Biochemistry, Genetics and Molecular Biology (all); Medicine (all)
Zito, Andrea; Bromuro, Carla; Mandili, Giorgia; Chiani, Paola; Horenstein, Alberto L.; Malavasi, Fabio; Cauda, Roberto; Cassone, Antonio; Torosantucci, Antonella
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1592842
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