Prosthetic joint infection diagnosis is often difficult since biofilm-embedded microorganisms attach well to the prosthetic surfaces and resist their detection by conventional methods. DL-dithiothreitol has been described as a valid method for biofilm detachment on orthopedic devices. We report the case of an occasional detection of Listeria monocytogenes in a non immuno-compromised patient with a preoperative diagnosis of aseptic loosening. The infection diagnosis due to such rare bacteria was made postoperatively, thanks to a DL-dithiothreitol-based device. This may be considered a feasible approach for the microbiological analysis of prosthetic joint infection, considering that a prompt diagnosis of such biofilmassociated infections could bring some advantages, such as an early and appropriate antibiotic therapy administration and a reduction of undiagnosed infections.

Unexpected Listeria monocytogenes detection with a dithiothreitol-based device during an aseptic hip revision [*G. Banche and A. Bistolfi contributed equally to this work; ** V. Allizond is the corresponding author]

G. Banche
First
;
V. Allizond
;
C. Merlino;A. Massè;A. M. Cuffini
Last
2018-01-01

Abstract

Prosthetic joint infection diagnosis is often difficult since biofilm-embedded microorganisms attach well to the prosthetic surfaces and resist their detection by conventional methods. DL-dithiothreitol has been described as a valid method for biofilm detachment on orthopedic devices. We report the case of an occasional detection of Listeria monocytogenes in a non immuno-compromised patient with a preoperative diagnosis of aseptic loosening. The infection diagnosis due to such rare bacteria was made postoperatively, thanks to a DL-dithiothreitol-based device. This may be considered a feasible approach for the microbiological analysis of prosthetic joint infection, considering that a prompt diagnosis of such biofilmassociated infections could bring some advantages, such as an early and appropriate antibiotic therapy administration and a reduction of undiagnosed infections.
2018
13
1295
1299
DL-dithiothreitol, Listeria monocytogenes , prosthetic joint infections
G. Banche*, A. Bistolfi*, V. Allizond**, C. Galletta, M.R. Iannantuoni, E.S. Marra, C. Merlino, A. Massè, A.M. Cuffini
File in questo prodotto:
File Dimensione Formato  
articolo preprint.pdf

Accesso aperto

Descrizione: articolo principale
Tipo di file: PREPRINT (PRIMA BOZZA)
Dimensione 1.14 MB
Formato Adobe PDF
1.14 MB Adobe PDF Visualizza/Apri
Banche_Fut Microbiol_2018.pdf

Accesso riservato

Descrizione: articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 2.03 MB
Formato Adobe PDF
2.03 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1678111
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 0
social impact