The capability of PCR-TTGE to detect meat species in mixed animal samples was investigated as a necessary step in developing a method where the identification will be performed matching on the “DNA barcode” zone the sequences of resolved PCR products obtained from a limited set of “universal” primers. Exemplary mixtures from five important meat species were analyzed. At this stage more PCR reactions have to be applied on a sample but this should be easily improved using primers simulta- neously (as a “cocktail”) in a single reaction.
Polymerase chain reaction products (PCR) on "DNA barcode zone" resolved by temporal temperature gradient electophoresis: A tool for species identification of mixed meat specimens - A technical note on preliminary results
Chessa S.;
2011-01-01
Abstract
The capability of PCR-TTGE to detect meat species in mixed animal samples was investigated as a necessary step in developing a method where the identification will be performed matching on the “DNA barcode” zone the sequences of resolved PCR products obtained from a limited set of “universal” primers. Exemplary mixtures from five important meat species were analyzed. At this stage more PCR reactions have to be applied on a sample but this should be easily improved using primers simulta- neously (as a “cocktail”) in a single reaction.
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