Aim: Growing evidence shows a strong interplay between post-transcriptional regulation, mediated by miRNAs (miRs) and epigenetic regulation. Nevertheless, the number of experimentally validated miRs (called epi-miRs) involved in these regulatory circuitries is still very small. Material & methods: We propose a pipeline to prioritize candidate epi-miRs and to identify potential epigenetic interactors of any given miR starting from miR transfection experiment datasets. Results & conclusion: We identified 34 candidate epi-miRs: 19 of them are known epi-miRs, while 15 are new. Moreover, using an in-house generated gene expression dataset, we experimentally proved that a component of the polycomb-repressive complex 2, the histone methyltransferase enhancer of zeste homolog 2 (EZH2), interacts with miR-214, a well-known prometastatic miR in melanoma and breast cancer, highlighting a miR-214-EZH2 regulatory axis potentially relevant in tumor progressio

Investigating the epi-miRNome: Identification of epi-miRNAs using transfection experiments

Reale E.;Taverna D.
Co-first
;
Cantini L.;Martignetti L.;Osella M.;Virga F.;Orso F.;Caselle M.
2019-01-01

Abstract

Aim: Growing evidence shows a strong interplay between post-transcriptional regulation, mediated by miRNAs (miRs) and epigenetic regulation. Nevertheless, the number of experimentally validated miRs (called epi-miRs) involved in these regulatory circuitries is still very small. Material & methods: We propose a pipeline to prioritize candidate epi-miRs and to identify potential epigenetic interactors of any given miR starting from miR transfection experiment datasets. Results & conclusion: We identified 34 candidate epi-miRs: 19 of them are known epi-miRs, while 15 are new. Moreover, using an in-house generated gene expression dataset, we experimentally proved that a component of the polycomb-repressive complex 2, the histone methyltransferase enhancer of zeste homolog 2 (EZH2), interacts with miR-214, a well-known prometastatic miR in melanoma and breast cancer, highlighting a miR-214-EZH2 regulatory axis potentially relevant in tumor progressio
2019
11
14
1581
1599
http://www.futuremedicine.com/loi/epi
epi-miRNA; epigenetic; EZH2; feedback loop; miR-138; miR-145; miR-214; miR-31; miRs; polycomb complex; tumor progression
Reale E.; Taverna D.; Cantini L.; Martignetti L.; Osella M.; De Pitta C.; Virga F.; Orso F.; Caselle M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1724081
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