[Preliminary serological analysis for identification of direct monoclonal antibodies against surface structures of HLA typed human lymphocytes]

The following schedule has been applied to the selection and preliminary serological definition of monoclonal antibodies against HLA controlled antigenic structures of human lymphocyte surface: 1) identification of antibodies reactive against the immunizing cells by means of indirect radiobinding technique and complement-dependent lymphocytotoxicity; 2) analysis of the pattern of reaction against a panel of T and B lymphocytes purified from peripheral blood of HLA typed donors. The reaction has been performed also against lymphocytes pretreated with anti-human-beta 2 microglobulin antibodies. Thus monomorphic and polymorphic monoclonal antibodies, directed against beta 2 microglobulin associated or not associated antigens, can be identified. The importance of the complement pool and of antibody avidity in the lymphocytotoxicity reaction mediated by monoclonal antibodies, is evaluated.