1. A simplified assay for the flux through the pentose phosphate and the Embden-Meyerhof pathways in the red cell is described. 2. [1-14C]glucose is metabolized to 14CO2 and 14C-anions. 3. 14CO2 is trapped with KOH and residual [14C]glucose and anions are separated by an enzymatic method, which is simpler and quicker than the usual chromatographic method.
A simplified method for the pentose phosphate pathway assay in red cells.
PESCARMONA, Gianpiero;BOSIA, Amalia;GHIGO, Dario Antonio
1982-01-01
Abstract
1. A simplified assay for the flux through the pentose phosphate and the Embden-Meyerhof pathways in the red cell is described. 2. [1-14C]glucose is metabolized to 14CO2 and 14C-anions. 3. 14CO2 is trapped with KOH and residual [14C]glucose and anions are separated by an enzymatic method, which is simpler and quicker than the usual chromatographic method.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
