Cell to cell interaction, which plays a crucial role in breast cancer growth, may be regulated by steroid hormones. This study examined dihydrotestosterone (DHT) effects on the interaction between the steroid receptor positive MCF-7 and the steroid receptor negative MDA-MB-231 breast cancer cell lines. The growth of MDA-MB-231 cells was inhibited by medium conditioned by MCF-7 cells grown in presence of DHT but not by medium conditioned by MCF-7 cells grown in presence of both DHT and the antiandrogen hydroxyflutamide. Trypsin pretreatment of conditioned medium abolished its growth-inhibitory effect on hormone-unresponsive cells. DHT itself did not affect the growth of MDA-MB-231 cells when directly added to their culture medium. Data suggest that DHT stimulates, via the androgen receptor, the androgen-responsive breast cancer cells to produce a peptide factor(s) capable of inhibiting the growth of hormone-unresponsive cells.

Dihydrotestosterone affects the growth of hormone-unresponsive breast cancer cells: an indirect action.

BOCCUZZI, Giuseppe
1995-01-01

Abstract

Cell to cell interaction, which plays a crucial role in breast cancer growth, may be regulated by steroid hormones. This study examined dihydrotestosterone (DHT) effects on the interaction between the steroid receptor positive MCF-7 and the steroid receptor negative MDA-MB-231 breast cancer cell lines. The growth of MDA-MB-231 cells was inhibited by medium conditioned by MCF-7 cells grown in presence of DHT but not by medium conditioned by MCF-7 cells grown in presence of both DHT and the antiandrogen hydroxyflutamide. Trypsin pretreatment of conditioned medium abolished its growth-inhibitory effect on hormone-unresponsive cells. DHT itself did not affect the growth of MDA-MB-231 cells when directly added to their culture medium. Data suggest that DHT stimulates, via the androgen receptor, the androgen-responsive breast cancer cells to produce a peptide factor(s) capable of inhibiting the growth of hormone-unresponsive cells.
1995
15
2581
2584
DI MONACO M ;LEONARDI L ;GATTO V ;GALLO M ;BRIGNARDELLO E ;BOCCUZZI G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/32035
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