Xenotransplantation is the possibile solution for the lacking of human tissues. Multiple problems exist including immunological reactions, metabolic differences between animals and humans and potential risks of the transmission of infections. Pigs are the most considered species for xenotransplantation. Numerous potential viruses have been identified including Rotavirus, Hepatitis E virus (HEV), Porcine Circovirus type 2 (PCV2), Porcine Herpesvirus and Porcine Endogenous Retrovirus (PERV). The aim of this study is to look for the presence of these viruses in different organs of n° 99 pigs healthy slaughtered in Piemonte region. Samples of small intestine, kidney, heart and inguinal lymph nodes were collected, fixed in 10% neutral buffered formalin for histological investigations or stored at –80°C for virological researches. Rotascreen Dipstick (Microgen Bioproducts), an immunochromatographic test from fecal specimens was used to identify the presence of Rotavirus. Electron microscopy was used to detect HEV-like morphological particles on faeces.

Xenotransplantation: potential presence of viral zoonosis in pig organs

SCAGLIONE, Frine Eleonora;CATALANO, DEBORAH;GUARDA, Franco;CAPUCCHIO, Maria Teresa
2006-01-01

Abstract

Xenotransplantation is the possibile solution for the lacking of human tissues. Multiple problems exist including immunological reactions, metabolic differences between animals and humans and potential risks of the transmission of infections. Pigs are the most considered species for xenotransplantation. Numerous potential viruses have been identified including Rotavirus, Hepatitis E virus (HEV), Porcine Circovirus type 2 (PCV2), Porcine Herpesvirus and Porcine Endogenous Retrovirus (PERV). The aim of this study is to look for the presence of these viruses in different organs of n° 99 pigs healthy slaughtered in Piemonte region. Samples of small intestine, kidney, heart and inguinal lymph nodes were collected, fixed in 10% neutral buffered formalin for histological investigations or stored at –80°C for virological researches. Rotascreen Dipstick (Microgen Bioproducts), an immunochromatographic test from fecal specimens was used to identify the presence of Rotavirus. Electron microscopy was used to detect HEV-like morphological particles on faeces.
2006
24th ESVP
EDINBURGH
31 agosto-2 settembre 2006
ESVP
ESVP
1
226
227
Scaglione F. E.; Catalano D.; Scrivano G.; Alborali L.; Ferrari M.; Villa R.; Appino S.; Guarda F.; Masoero L.; Capucchio M.T.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/52165
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