The aim of this research was to evaluate the use of cyclodextrin-based nanospugnes (NS), complexed with plant growth regulators, to optimize micropropagation of 4 cultivars of Camellia japonica L. ('California', 'General Coletti', 'Doctor Burnside', and 'Charles Cobb'). Apical shoots were placed in Anderson's Rhodondendron (AR) multiplication medium, supplemented with 1 mg L-1 benzyladenine (BA),. The explants were kept in climatic chamber at 25 °C and photoperiod 12h/8h. The highest multiplication rates were found in 'General Coletti' and 'Doctor Burnside' (39% and 34%, respectively), while in 'California' and 'Charles Cobb' they were nearly halved (18% and 19%, respectively). The shoots were subsequently transferred to a rooting AR substrate, added with indole-3-butyric acid (IBA, 0.5 and 1 mg L-1), included or not in β-NS 1:4. A second experiment was carried out maintaining the shoots on substrate enriched with IBA, included or not in β-NS 1:4 for 11 days to induce the differentiation of root primordia and then transferred to AR basal medium. In both experiments, the explants were kept in the climatic chamber for 18 days in the dark at 18 °C temperature, and then at 25 °C during the day and 18°C during the night, associated to photoperiod 12h/12h. After 11 months, data on rooting are still not available.

Studio sulla moltiplicazione in vitro di Camellia japonica e prime valutazioni delle potenzialità d’impiego di nanospugne nell’ambito di processi di radicazione

SEGLIE, LUDOVICA;SCARIOT, VALENTINA;TROTTA, Francesco;DEVECCHI, Marco
2011-01-01

Abstract

The aim of this research was to evaluate the use of cyclodextrin-based nanospugnes (NS), complexed with plant growth regulators, to optimize micropropagation of 4 cultivars of Camellia japonica L. ('California', 'General Coletti', 'Doctor Burnside', and 'Charles Cobb'). Apical shoots were placed in Anderson's Rhodondendron (AR) multiplication medium, supplemented with 1 mg L-1 benzyladenine (BA),. The explants were kept in climatic chamber at 25 °C and photoperiod 12h/8h. The highest multiplication rates were found in 'General Coletti' and 'Doctor Burnside' (39% and 34%, respectively), while in 'California' and 'Charles Cobb' they were nearly halved (18% and 19%, respectively). The shoots were subsequently transferred to a rooting AR substrate, added with indole-3-butyric acid (IBA, 0.5 and 1 mg L-1), included or not in β-NS 1:4. A second experiment was carried out maintaining the shoots on substrate enriched with IBA, included or not in β-NS 1:4 for 11 days to induce the differentiation of root primordia and then transferred to AR basal medium. In both experiments, the explants were kept in the climatic chamber for 18 days in the dark at 18 °C temperature, and then at 25 °C during the day and 18°C during the night, associated to photoperiod 12h/12h. After 11 months, data on rooting are still not available.
2011
2
105
107
arbustive species; inclusion compounds; phytohormones
Seglie L; Scariot V; Trotta F; Devecchi m
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/98004
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