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8:00
#PS 15 / IL
Applications of Novel Imaging Techniques (#656) Jan-Bernd H�vener
Medical Physics, Dept. Radiology University Medical Center Freiburg, Breisacher Stra�e 60a, Freiburg 79098, Germany
8:25
#PS 15 / 1
Multimodal
non-invasive longitudinal studies of imaging biomarkers for the
quantification of colon inflammation in a mouse model of colitis (#361)
Anne Beltzer 1, Andrea Bianchi 1, Teresa Bluhmki 1, Tanja Schoenberger 2, Andrea V�gtle 1, David Kind 1, Eric Kaaru 1, Michael Neumaier 1, Birgit Stierstorfer 2, Thomas Kaulisch 1, Detlef Stiller 1 1Boehringer Ingelheim Pharma GmbH & Co. KG Target
Discovery Research, In-vivo imaging laboratory, Biberach an der Riss,
Germany 2Boehringer Ingelheim Pharma GmbH & Co.
KG Target Discovery Research, Target Validation Technologies, Biberach
an der Riss, Germany
8:37
#PS 15 / 2
Phase contrast CT for quantification of structural changes in lungs of asthma mouse models of different severity (#176) Christian Dullin 1, Emanuel Larsson 2,3,4, Giuliana Tromba 2, Andrea Markus 5, Frauke Alves 5,1,6 1University Medical Hospital Goettingen Diagnostic and
Interventional Radiology, Robert Koch Str. 40, 37075 Goettingen, Germany
2Elettra-Sincrotrone Trieste SYRMEP beamline, Strada Statale 14 - km 163,5 in AREA Science Park, 34149 Trieste, Italy 3University of Trieste Architecture and Engineering, 34149 Trieste, Italy 4Link�ping University Physics, Chemistry and Biology, SE-58183 Linkoeping, Sweden 5University Medical Hospital Goettingen Haematology and medical Oncology, Robert Koch Str. 40, 37075 Goettingen, Germany 6Max
Plank Institute for experimental Medicine Molecular Biology of neuronal
Signals, Hermann-Rein-Str. 3, 37075 Goettingen, Germany
Introduction Lung
imaging in mouse disease models is crucial for the assessment of the
severity of airway disease but remains challenging due to the small size
and the high porosity of the organ. Synchrotron inline free propagation
phase contrast CT with its intrinsic high soft-tissue contrast provides
the necessary sensitivity and spatial resolution to analyse the mouse
lung structure in great detail.
Methods We
analysed the benefit of this technique in combination with single
distance phase retrieval to quantify alterations of the lung structure
in asthma mouse models of different severity. In order to mimic an in-vivo situation
as close as possible, the lungs were inflated with air at a constant
physiological pressure. Entire mice were embedded in agarose gel and
imaged using inline free propagation phase contrast CT at the SYRMEP
beamline (Synchrotron Light Source, �Elettra�, Trieste, Italy).
Results The
quantification of the obtained phase contrast CT data sets revealed an
increasing lung soft-tissue content in mice correlating with the degree
of asthma severity (Fig. 1). In addition, we found significant changes
in the real part of the complex refractive index pointing to a
modification of the lung soft tissue composition. Interestingly, these
changes differ in between mild and severe acute asthma and therefore may
reflect variations in the underlying pathomechanism. Based on these
findings, it was possible to successfully discriminate between healthy
controls and mice with either mild or severe asthma.
Conclusions We
believe that our approach may have the potential to evaluate the
efficacy of novel therapeutic strategies that have effects on the airway
remodelling processes in asthma.
Acknowledgement This
work was supported by the German Research Foundation (Deutsche
Forschungsgemeinschaft, DFG) [DU 1403/1-1] and by the COST MP1207
action. The authors thank Sarah Greco and B�rbel Heidrich for their
excellent work of setting up the asthma models used in the study. In
addition, we thank the whole team of the SYRMEP beamline which has
contributed to this work in many different ways; especially we thank
Nicola Sodini for his technical assistance and essential support for the
success of the study. Also, we thank the group of Tim Gureyev who
provided the software �X-tract�, used to perform the phase retrieval of
the data sets.
Fig. 1:
VR of a control (CN), mild asthmatic (MAA) and severe asthmatic
(SAA) mouse shows increasing soft-tissue content in the lung in
correlation with increasing asthma severity.
Multiparametric characterization of kidney function and perfusion exploiting a dynamic CEST approach (#37) Dario Longo 1,2, Lorena Consolino 3,2, Pietro Irrera 3, Juan Carlos Cutrin 3,2, Silvio Aime 3,2 1Institute of Biostructure and Bioimaging - CNR Molecular Biotechnolgy and Health Sciences, Via Nizza, 52, 10126 Torino, Italy 2University of Torino Molecular Imaging Center, Via Nizza, 52, 10126 Torino, Italy 3University of Torino Molecular Biotechnolgy and Health Sciences, Via Nizza, 52, 10126 Torino, Italy
9:01
#PS 15 / 5
Hyperpolarization using Parahydrogen of biologically relevant substrates: acetate and pyruvate (#450) Francesca Reineri 1, Tommaso Boi 2, Silvio Aime 1 1University of Torino Molecular biotechnology and health sciences, Via Nizza 52, 10123 Torino, Italy 2Bracco Imaging Spa, CRB, 10010 Colleretto Giacosa (TO), Italy
Introduction Hyperpolarization methods represent a recent break-through in field of diagnostic tools by Magnetic Resonance. The in vivo administration of 13C-labelled
metabolites, hyperpolarized by means of DNP (Dynamic Nuclear
Polarization), can provide outstanding information about intracellular
metabolism. Parahydrogen Induced Polarization (PHIP) is an
alternative route to hyperpolarization that has the advantage of being
cheaper and easier to handle. The application of PHIP to bio-medical
studies has been limited by the availability of unsaturated precursors.
Our work shows how parahydrogen can be used to obtain hyperpolarization
on some biologically relevant molecules such as pyruvate and acetate.
Methods In order to obtain hyperpolarization on the 13C
carboxylate signal of carboxylic acids such as acetate and pyruvate, an
ester is synthesized in which the unsaturated bond is placed on the
alcoholic moiety. The vinyl ester of acetate, propargyl esters of
acetate and pyruvate are used. Parahydrogenation is carried out in both
organic solvent and aqueous phase, using a Rh(I) catalyst and 92%
enriched parahydrogen. Magnetic field cycling is applied to achieve
polarization transfer from parahydrogen spin order to 13C net magnetization. Hydrolysis of the polarized ester is carried out using NaOD (1M).
Results Parahydrogenation
of vinyl and propargyl esters of acetate, followed by magnetic field
cycling, allows achieving hyperpolarization of the 13C carboxylate signal. Hydrolysis of the parahydrogenated esters leads to 13C hyperpolarized Sodium acetate (figure 1). Parahydrogenation
of propargylic ester of pyruvate in organic medium (chloroform/methanol
mixture) and application of field cycling allows polarization transfer
to the 1-13C carboxylate signal. After the polarization transfer step,
hydrolysis is carried out by the addition of an aqueous basic solution
and [1-13C] hyperpolarized pyruvate is obtained in the aqueous phase (figure 2).
Conclusions The experimental results show that high polarization level can be obtained on the 13C
carboxylate signal of parahydrogenated esters on which parahydrogen is
added to the alcoholic moiety. Theoretical studies allow to demonstrate
that field cycling allows to obtain a polarization level on the 13C
carboxylate signal which is as high as that observed on molecules where
parahydrogen is added at adjacent positions to the target 13C nucleus. The
herein presented method, namely ParaHydrogen Induced Polarization by
means of Side Arm Hydrogenation (PHIP-SAH), markedly widens the
applicability of the PHIP approach for the hyperpolarization of
biologically relevant molecules.
Figure 1:
a) hyperpolarized carboxylate signal of parahydrogenated ethyl
acetate (naturally abundant 13C); b) after hydrolysis, hyperpolarized
Sodium acetate is obtained. Both spectra are single scan. c) thermal
signal, 1000 scans, 56h acquisition.
Figure 2:
a) hyperpolarized 13C signals of parahydrogenated
allyl-pyruvate, the asterisk indicates the acetalic form of pyruvate
obtained in the organic phase; b) after hydrolysis hyperpolarized Sodium
pyruvate is obtained. c) thermal equilibrium 13C NMR spectrum.
Il report seguente simula gli indicatori relativi alla produzione scientifica in relazione alle soglie ASN 2023-2025 del proprio SC/SSD. Si ricorda che il superamento dei valori soglia (almeno 2 su 3) è requisito necessario ma non sufficiente al conseguimento dell'abilitazione.
La simulazione si basa sui dati IRIS e presenta gli indicatori calcolati alla data indicata sul report. Si ricorda che in sede di domanda ASN presso il MIUR gli indicatori saranno invece calcolati a partire dal 1° gennaio rispettivamente del quinto/decimo/quindicesimo anno precedente la scadenza del quadrimestre di presentazione della domanda (art 2 del DM 598/2018).
In questa simulazione pertanto il valore degli indicatori potrà differire da quello conteggiato all’atto della domanda ASN effettuata presso il MIUR a seguito di:
Correzioni imputabili a eventuali periodi di congedo obbligatorio, che in sede di domanda ASN danno diritto a incrementi percentuali dei valori.
Presenza di eventuali errori di catalogazione e/o dati mancanti in IRIS
Variabilità nel tempo dei valori citazionali (per i settori bibliometrici)
Variabilità della finestra temporale considerata in funzione della sessione di domanda ASN a cui si partecipa.
La presente simulazione è stata realizzata sulla base delle regole riportate nel DM 598/2018 e dell'allegata Tabella A e delle specifiche definite all'interno del Focus Group Cineca relativo al modulo IRIS ER. Il Cineca non si assume alcuna responsabilità in merito all'uso che il diretto interessato o terzi faranno della simulazione.
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