Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface-enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.
SERS active Ag nanoparticles in mesoporous silicon: detection of organic molecules and peptide-antibody assays
SOSTER, MARCO;BUSSOLINO, Federico;MARCHIO', Serena;
2012-01-01
Abstract
Ag nanoparticles synthesized on porous silicon samples were studied and applied as surface-enhanced Raman scattering (SERS) substrates. SERS activity was tested using Cy5 dye verifying an enhancement factor up to 107. CGIYRLRS peptides were chemisorbed on the Ag nanoparticles with the plasmonic resonance tuned at the excitation energy. Such oligopeptides were used as baits for a specific polyclonal antibody. The overall Raman enhancement allowed to evidence a good selectivity to the target analyte as required by most of the SERS applications on biological assays.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
