CINECA IRIS Institutional Research Information System
IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Torino.
AperTO è l'archivio istituzionale Open Access destinato a raccogliere, rendere visibile e conservare la produzione scientifica dell'Università degli Studi di Torino.
EnglishCholesterol oxidation products are increasingly considered as much more bioactive than the parent compound in the mutifactored and multistep process which characterizes atherosclerosis. In particular, 7-ketocholesterol has been reported to induce oxidative stress as well as a marked pro-apoptotic effect in vascular cells including macrophages. With the aim to investigate a possible pathogenic correlation between the two events, cultivated murine macrophages were challenged with a concentration of 7-ketocholesterol actually detectable in human vasculature. Conclusive proof was obtained of a primary role of NADPH-oxidase in the overproduction of reactive oxygen species within cells treated with the oxysterol. In addition, such oxidative burst occurred very early after cell intoxication and it was definitely demonstrated as able to lead cells to apoptotic death. In fact, two metabolic inhibitors of NADPH-oxidase and the antioxidant epicatechin very well counteracted 7-ketocholesterol-induced apoptosis by preventing the oxysterol pro-oxidant action.
Early involvement of ROS overproduction in apoptosis induced by 7-ketocholesterol. / Leonarduzzi G; Vizio B; Sottero B; Verde V; Gamba P; Mascia C; Chiarpotto E; Poli G; Biasi F. - In: ANTIOXIDANTS & REDOX SIGNALING. - ISSN 1523-0864. - 8(2006), pp. 375-380.
| Titolo: | Early involvement of ROS overproduction in apoptosis induced by 7-ketocholesterol. |
| Autori Riconosciuti: | |
| Autori: | Leonarduzzi G; Vizio B; Sottero B; Verde V; Gamba P; Mascia C; Chiarpotto E; Poli G; Biasi F |
| Data di pubblicazione: | 2006 |
| Abstract: | Cholesterol oxidation products are increasingly considered as much more bioactive than the parent compound in the mutifactored and multistep process which characterizes atherosclerosis. In particular, 7-ketocholesterol has been reported to induce oxidative stress as well as a marked pro-apoptotic effect in vascular cells including macrophages. With the aim to investigate a possible pathogenic correlation between the two events, cultivated murine macrophages were challenged with a concentration of 7-ketocholesterol actually detectable in human vasculature. Conclusive proof was obtained of a primary role of NADPH-oxidase in the overproduction of reactive oxygen species within cells treated with the oxysterol. In addition, such oxidative burst occurred very early after cell intoxication and it was definitely demonstrated as able to lead cells to apoptotic death. In fact, two metabolic inhibitors of NADPH-oxidase and the antioxidant epicatechin very well counteracted 7-ketocholesterol-induced apoptosis by preventing the oxysterol pro-oxidant action. |
| Volume: | 8 |
| Pagina iniziale: | 375 |
| Pagina finale: | 380 |
| Rivista: | ANTIOXIDANTS & REDOX SIGNALING |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| ARS 2006.pdf | POSTPRINT (VERSIONE FINALE DELL’AUTORE) | Accesso riservato | Utenti riconosciuti Richiedi una copia |
http://hdl.handle.net/2318/101678
Copyright © 2021