A simplified method to detect faecal sterols, as an alternative assessment of environmental faecal pollution is proposed. The aim of this study is the development of a method to determine sterols in water samples avoiding sample filtration through glass fibre filter. The method is based on a liquid–liquid extraction and a final GC–FID determination. The quantified sterols are coprostanol and 24-ethylcoprostanol, while 5-cholestane is used as internal standard. The recovery of coprostanol and 24-ethylcoprostanol in wastewater ranges from 90 to 100% and the detection limit is 1–2g l−1. Moreover the method proved to be useful for the sterols determination in surface water too.

Faecal sterols determination in wastewater and surface water

GILLI, Giorgio;ROVERE, Renato;TRAVERSI, Deborah;SCHILIRO', Tiziana;PIGNATA, Cristina
2006

Abstract

A simplified method to detect faecal sterols, as an alternative assessment of environmental faecal pollution is proposed. The aim of this study is the development of a method to determine sterols in water samples avoiding sample filtration through glass fibre filter. The method is based on a liquid–liquid extraction and a final GC–FID determination. The quantified sterols are coprostanol and 24-ethylcoprostanol, while 5-cholestane is used as internal standard. The recovery of coprostanol and 24-ethylcoprostanol in wastewater ranges from 90 to 100% and the detection limit is 1–2g l−1. Moreover the method proved to be useful for the sterols determination in surface water too.
JOURNAL OF CHROMATOGRAPHY. B
843 (1)
120
124
GILLI G; ROVERE R; TRAVERSI D; SCHILIRO' T; PIGNATA C
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/102509
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