Aim. The aim of this study was to investigate the subset distribution and proliferative response of peripheral blood (PB) T cells in a group of pregnant women who experienced previous unexplained recurrent spontaneous abortion (RSA) in comparison with a control group of healthy pregnant women. Methods. Twenty women with RSA and 25 fertile women in the 1st trimester of pregnancy were included in the study. Activation antigens of peripheral T lymphocytes and their proliferative response were compared. The PB lymphocytes were stained with monoclonal antibodies. The in vitro T cells proliferation analyses were performed by stimulation with anti-CD3, interleukin-2 and phytohemagglutinin. Results. Percentage and absolute number of T CD4+ lymphocytes were significantly higher in patients with RSA than in fertile pregnant women. Analysis of CD4+ T cells subpopulation showed that RSA patients displayed a lower proportion of CD25+ cells and a lower expression of H4/ICOS activation antigen. T cells from patients with RSA display a decreased proliferative response to all the stimuli used. Conclusion. These data underline that an altered modulation of maternal CD4+ T cell population is associated with unexplained recurrent abortion

Phenotypic and functional analyses of peripheral blood T cells in women with recurrent spontaneous abortion in early stages of pregnancy

MENATO, Guido;PATRIARCA, Ambra;BOCCADORO, Mario;BENEDETTO, Chiara;MASSOBRIO, Marco
2007-01-01

Abstract

Aim. The aim of this study was to investigate the subset distribution and proliferative response of peripheral blood (PB) T cells in a group of pregnant women who experienced previous unexplained recurrent spontaneous abortion (RSA) in comparison with a control group of healthy pregnant women. Methods. Twenty women with RSA and 25 fertile women in the 1st trimester of pregnancy were included in the study. Activation antigens of peripheral T lymphocytes and their proliferative response were compared. The PB lymphocytes were stained with monoclonal antibodies. The in vitro T cells proliferation analyses were performed by stimulation with anti-CD3, interleukin-2 and phytohemagglutinin. Results. Percentage and absolute number of T CD4+ lymphocytes were significantly higher in patients with RSA than in fertile pregnant women. Analysis of CD4+ T cells subpopulation showed that RSA patients displayed a lower proportion of CD25+ cells and a lower expression of H4/ICOS activation antigen. T cells from patients with RSA display a decreased proliferative response to all the stimuli used. Conclusion. These data underline that an altered modulation of maternal CD4+ T cell population is associated with unexplained recurrent abortion
2007
19
75
82
CASTAGNA P; REDOGLIA V; STEFANIDOU M; MENATO G; PATRIARCA A; BOCCADORO M; BENEDETTO C; MASSOBRIO M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/104764
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