Isolation, culture and characterization of embryonic cell lines from vitrified sheep blastocysts

Isolation, culture and characterization of embryonic cell lines from vitrified sheep blastocysts derived from in vitro production. In 5/6 days the inner cells mass cultured on mice STo fibroblasts are disaggregated , seeded in a new MIF to form new colonies. After 3/4 days the cells were disggregate and re cultivated for another 3/4 days. The different cells expressed stage specific embryonic markers SSEA-1 SSEA-3 SSEA-4 and were alkaline phophatase positive. In absence of feeder layer and LIF these cells differentiated into variety off cell type and embryoid bodies.