Abstract The preparation and characterization of PCL guides filled with a porous matrix of a freeze-dried, genipin-crosslinked gelatin sponge (GL/GP) is described. Porous guides are obtained by dip coating a rotating mandrel with a PCL/PEO blend solution followed by PEO solvent extraction. PCL/PEO 60/40 is selected as the optimal composition to obtain model porous membranes with suitable morphological properties to ensure nutrient permeation and moderate stiffness. GL/GP sponges show increased mechanical strength and water stability, compared to uncrosslinked GL sponges. GL/GP in the form of films, sponges and internal fillers support the in vitro adhesion and proliferation of Schwann like cells.
Porous poly(îμ-caprolactone) nerve guide filled with porous gelatin matrix for nerve tissue engineering.
AUDISIO, Chiara;GNAVI, SARA;RAIMONDO, Stefania;GEUNA, Stefano;PERROTEAU, Isabelle;
2011-01-01
Abstract
Abstract The preparation and characterization of PCL guides filled with a porous matrix of a freeze-dried, genipin-crosslinked gelatin sponge (GL/GP) is described. Porous guides are obtained by dip coating a rotating mandrel with a PCL/PEO blend solution followed by PEO solvent extraction. PCL/PEO 60/40 is selected as the optimal composition to obtain model porous membranes with suitable morphological properties to ensure nutrient permeation and moderate stiffness. GL/GP sponges show increased mechanical strength and water stability, compared to uncrosslinked GL sponges. GL/GP in the form of films, sponges and internal fillers support the in vitro adhesion and proliferation of Schwann like cells.
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