Aquaporin-4 (AQP-4), the most important water channel in the brain, is expressed by astrocyte endfeet abutting microvessels. Altered expression levels of AQP-4 and redistribution of the protein throughout the membranes of cells found in glioblastoma multiforme (GBM) lead to development of the oedema often found surrounding the tumour mass. Dysregulation of AQP-4 also occurs in hippocampal sclerosis and cortical dysplasia in patients with refractory partial epilepsy. This work reports on analysis of the relationship between AQP-4 expression and the incidence of epileptic seizures in patients with GBM. Immunohistochemical and PCR techniques were used to evaluate AQP-4 in biopsy specimens from 19 patients with GBM, 10 of whom had a history of seizures prior to surgery. AQP-4 mRNA levels were identical in the two groups of patients, but AQP-4 expression was more frequently detected on the GBM membranes from specimens of patients with seizures than from those without (10 vs. 2, P<p:0.001). We conclude that reduced expression of cell surface AQP-4 is characteristic of GBM patients without seizures, likely attributable to a post-translational mechanism

DIFFERENT AQUAPORIN-4 EXPRESSION IN GLIOBLASTOMA MULTIFORME PATIENTS WITH AND WITHOUT SEIZURES.

AUDRITO, VALENTINA;DEAGLIO, Silvia;MALAVASI, Fabio;
2012

Abstract

Aquaporin-4 (AQP-4), the most important water channel in the brain, is expressed by astrocyte endfeet abutting microvessels. Altered expression levels of AQP-4 and redistribution of the protein throughout the membranes of cells found in glioblastoma multiforme (GBM) lead to development of the oedema often found surrounding the tumour mass. Dysregulation of AQP-4 also occurs in hippocampal sclerosis and cortical dysplasia in patients with refractory partial epilepsy. This work reports on analysis of the relationship between AQP-4 expression and the incidence of epileptic seizures in patients with GBM. Immunohistochemical and PCR techniques were used to evaluate AQP-4 in biopsy specimens from 19 patients with GBM, 10 of whom had a history of seizures prior to surgery. AQP-4 mRNA levels were identical in the two groups of patients, but AQP-4 expression was more frequently detected on the GBM membranes from specimens of patients with seizures than from those without (10 vs. 2, P
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glioblastoma; aquaporin
Isoardo G; Morra I; Chiarle G; Audrito V; Deaglio S; Melcarne A; Junemann C; Naddeo M; Cogoni M; Valentini MC; Limberti A; Faccani F; Malavasi F; Faccani G.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/115686
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