The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of a-trimethylglutamine (NMe3Gln), a-trimethylglutamate (NMe3Glu) and e-trimethyllysine (NMe3Lys) are reported. The 15N-permethylated, perdeuterated amino acids display very long 15N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP 15N-MRI applications such as perfusion or targeting studies
15N-Permethylated amino acids as efficient probes for MRI-DNP applications
CHIAVAZZA, ENRICO;VIALE, Alessandra;AIME, Silvio
2013-01-01
Abstract
The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of a-trimethylglutamine (NMe3Gln), a-trimethylglutamate (NMe3Glu) and e-trimethyllysine (NMe3Lys) are reported. The 15N-permethylated, perdeuterated amino acids display very long 15N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP 15N-MRI applications such as perfusion or targeting studies
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