Callus cultures, obtained from Nicotiana tabacum and from two biotypes (atrazine-resistant and susceptible) of Solanum nigrum, were cultured in the presence of atrazine (0 - 35 - 70 uM). Herbicide effects were observed with two sucrose levels (2.5 and 10 g/L) and evaluated by dry weight measurements. The higher sucrose concentration assured a greater growth of the cultures. Atrazine, with both sugar levels, selectively affected the callus proliferation: susceptible cultures bleached within 15 days and were unable to grow, in contrast with the resistant ones, which maintained a green colour and grew similar to the untreated calli. It is possible that growth inhibition depend on secondary effects following the interruption of electon flow at the photosystem II level.
Selective action of atrazine in callus cultures of Solanum nigrum and Nicotiana tabacum.
DE DONATO, Mariano;MOZZETTI MONTERUMICI, Chiara;PERUCCO, Ezio
1990-01-01
Abstract
Callus cultures, obtained from Nicotiana tabacum and from two biotypes (atrazine-resistant and susceptible) of Solanum nigrum, were cultured in the presence of atrazine (0 - 35 - 70 uM). Herbicide effects were observed with two sucrose levels (2.5 and 10 g/L) and evaluated by dry weight measurements. The higher sucrose concentration assured a greater growth of the cultures. Atrazine, with both sugar levels, selectively affected the callus proliferation: susceptible cultures bleached within 15 days and were unable to grow, in contrast with the resistant ones, which maintained a green colour and grew similar to the untreated calli. It is possible that growth inhibition depend on secondary effects following the interruption of electon flow at the photosystem II level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.