Genetic resources can be described as the total genetic diversity of cultivated species and their wild relatives. Their effective conservation and use depend on the availability of information and on the extent and distribution of genetic diversity in species of interest. In recent years, numerous molecular techniques have been developed to provide markers. Among the several classes of DNA-based markers, the Sequence Tagged Microsatellite Sites (STMS) are highly polymorphic, multi-allelic, frequently co-dominant, highly reproducible, selectively neutral, and widely and randomly distributed in the genome. In order to improve the genetic knowledge in ornamentals and to solve taxonomical issues, STMSs were used to investigate the Camellia genus. A set of 44 cultivars belonging to C. sasanqua L., C. × hiemalis Nakai, C. hybrid, and C. × vernalis Makino were characterised by means of 4 STMSs and 15 morphological traits referring to flower and leaf morphology, colour, and fragrance. STMS markers successfully amplified all the accessions, and 58 alleles were scored. The distribution of the genetic variation highlighted genetic overlapping among C. sasanqua cultivars and the hybrids of C. × vernalis, C. × hiemalis, and C. hybrid. The Principal Coordinate Analyses (PCoA) based on joined molecular and morphological data sets showed a distribution of all accessions in agreement with their taxonomic classification. Taken as a whole, results demonstrated that this set of microsatellite loci are suitable for describing genetic diversity in fall-blooming camellias, and thus constitute an appropriate tool for germplasm characterization and management.
CHARACTERISATION OF FALL-BLOOMING CAMELLIAS AS REVEALED BY SEQUENCE TAGGED MICROSATELLITE SITE MARKERS AND MORPHOLOGICAL TRAITS
CASER, Matteo;SCARIOT, VALENTINA
2011-01-01
Abstract
Genetic resources can be described as the total genetic diversity of cultivated species and their wild relatives. Their effective conservation and use depend on the availability of information and on the extent and distribution of genetic diversity in species of interest. In recent years, numerous molecular techniques have been developed to provide markers. Among the several classes of DNA-based markers, the Sequence Tagged Microsatellite Sites (STMS) are highly polymorphic, multi-allelic, frequently co-dominant, highly reproducible, selectively neutral, and widely and randomly distributed in the genome. In order to improve the genetic knowledge in ornamentals and to solve taxonomical issues, STMSs were used to investigate the Camellia genus. A set of 44 cultivars belonging to C. sasanqua L., C. × hiemalis Nakai, C. hybrid, and C. × vernalis Makino were characterised by means of 4 STMSs and 15 morphological traits referring to flower and leaf morphology, colour, and fragrance. STMS markers successfully amplified all the accessions, and 58 alleles were scored. The distribution of the genetic variation highlighted genetic overlapping among C. sasanqua cultivars and the hybrids of C. × vernalis, C. × hiemalis, and C. hybrid. The Principal Coordinate Analyses (PCoA) based on joined molecular and morphological data sets showed a distribution of all accessions in agreement with their taxonomic classification. Taken as a whole, results demonstrated that this set of microsatellite loci are suitable for describing genetic diversity in fall-blooming camellias, and thus constitute an appropriate tool for germplasm characterization and management.
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