Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300 nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327 ± 45 nm and 1.64 ± 0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).

Crosslinked gelatin nanofibres: Preparation, characterisation and in vitro studies using glial-like cells

CIPRIANI, ELISA;GNAVI, SARA;PERROTEAU, Isabelle;ZANETTI, Marco;
2013-01-01

Abstract

Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300 nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327 ± 45 nm and 1.64 ± 0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).
2013
33
5
2723
2735
http://www.sciencedirect.com/science/article/pii/S0928493113001392
Electrospinning; Gelatin; Nanofibres; Aqueous solution; Glial cells
C. Tonda-Turo; E. Cipriani; S. Gnavi; V. Chiono; C. Mattu; P. Gentile; I. Perroteau; M. Zanetti; G. Ciardelli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/133319
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