Nanometric TiO2 has been reported to be cytotoxic and genotoxic in different in vitro models when activated by UV light. However, a clear picture of the species mediating the observed toxic effects is still missing. Here, a nanometric TiO2 powder has been modified at the surface to completely inhibit its photo-catalytic activity and to inhibit the generation of all reactive species except for singlet oxygen. The prepared powders have been tested for their ability to induce strand breaks in plasmid DNA and for their cytotoxicity and genotoxicity toward human keratinocyte (HaCaT) cells (100–500 μg mL−1, 15 min UVA/B exposure at 216–36 mJ m−2 respectively). The data reported herein indicate that the photo-toxicity of TiO2 is mainly triggered by particle-derived singlet oxygen. The data presented herein contribute to the knowledge of structure–activity relationships which are needed for the design of safe nanomaterials.

Singlet oxygen plays a key role in the toxicity and DNA damage of nanometric TiO2 to human keratinocytes

FENOGLIO, Ivana;GHIAZZA, Mara;CORAZZARI, INGRID;OLIARO BOSSO, Simonetta;
2013-01-01

Abstract

Nanometric TiO2 has been reported to be cytotoxic and genotoxic in different in vitro models when activated by UV light. However, a clear picture of the species mediating the observed toxic effects is still missing. Here, a nanometric TiO2 powder has been modified at the surface to completely inhibit its photo-catalytic activity and to inhibit the generation of all reactive species except for singlet oxygen. The prepared powders have been tested for their ability to induce strand breaks in plasmid DNA and for their cytotoxicity and genotoxicity toward human keratinocyte (HaCaT) cells (100–500 μg mL−1, 15 min UVA/B exposure at 216–36 mJ m−2 respectively). The data reported herein indicate that the photo-toxicity of TiO2 is mainly triggered by particle-derived singlet oxygen. The data presented herein contribute to the knowledge of structure–activity relationships which are needed for the design of safe nanomaterials.
2013
Inglese
Esperti anonimi
5
14
6567
6576
10
Nanoparticles; Titania; Singlet oxygen; Genotoxicity; Photoactivity; Sunscreens
no
1 – prodotto con file in versione Open Access (allegherò il file al passo 6 - Carica)
262
9
Ivana Fenoglio; Jessica Ponti; Elisa Alloa; Mara Ghiazza; Ingrid Corazzari; Robin Capomaccio; Diana Rembges; Simonetta Oliaro-Bosso; François Rossi...espandi
info:eu-repo/semantics/article
open
03-CONTRIBUTO IN RIVISTA::03A-Articolo su Rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/136715
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