This study was aimed at evaluating the effect of prolonged refrigeration of expressed human milk on its protein and microbial profile. Human milk from mothers of pre-term newborns was collected, pooled and stored in the Neonatal Intensive Care Unit refrigerator. Pooled milk was aliquoted and analysed within 96 h. The milk samples were analysed for pH, total protein content, protein profile, secretory immunoglobulin A (sIgA), available lysine and lactose contents, and bacterial profile (total aerobic bacterial count, Enterobacteriaceae, coagulase-positive Staphylococci, lactic acid bacteria). The refrigeration process did not affect the abundance of single proteins, nor the available lysine and sIgA contents. Lactose and pH showed a significant, although limited, decrease. The main microbial communities of human milk were preserved during refrigerated storage. Important markers of milk nutritional quality and bioactive proteins, including the antimicrobial components lysozyme and lactoferrin, as well as immunological components, were preserved.

Effect of prolonged refrigeration on the protein and microbial profile of human milk

GASTALDI, Daniela;PEILA, Chiara;Coscia A;BERTINO, Enrico
2013-01-01

Abstract

This study was aimed at evaluating the effect of prolonged refrigeration of expressed human milk on its protein and microbial profile. Human milk from mothers of pre-term newborns was collected, pooled and stored in the Neonatal Intensive Care Unit refrigerator. Pooled milk was aliquoted and analysed within 96 h. The milk samples were analysed for pH, total protein content, protein profile, secretory immunoglobulin A (sIgA), available lysine and lactose contents, and bacterial profile (total aerobic bacterial count, Enterobacteriaceae, coagulase-positive Staphylococci, lactic acid bacteria). The refrigeration process did not affect the abundance of single proteins, nor the available lysine and sIgA contents. Lactose and pH showed a significant, although limited, decrease. The main microbial communities of human milk were preserved during refrigerated storage. Important markers of milk nutritional quality and bioactive proteins, including the antimicrobial components lysozyme and lactoferrin, as well as immunological components, were preserved.
2013
31
2
121
126
Enterobacteriaceae, Lactic acid bacteria, Microbial communities, Microbial profiles, Neonatal intensive care units, Nutritional qualities, Refrigerated storages, Total protein contents
Giribaldi M; Ortoffi MF; Giuffrida MG; Gastaldi D; Peila C; Coscia A; Raia M; Arslanoglu S; Moro GE; Cavallarin L; Bertino E
File in questo prodotto:
File Dimensione Formato  
2013 Protein Refrigeration.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 254.38 kB
Formato Adobe PDF
254.38 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/139439
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 13
social impact