Salvia officinalis L. (Sage) is an important industrial plant used both for food and pharmaceutical purposes. The terpene fraction of this plant, which contains thujones and other mono and sesquiterpenoids, is responsible for many of its therapeutic and culinary properties. We used two extraction methods [hydrodistillation (HD) to obtain the essential oil (EO), and supercritical CO2 extraction (SFE)] to analyze by gas chromatography–mass spectrometry (GC–MS) the terpene fraction extracted from sage dried leaves. α-Thujone, β-thujone and other oxygenated monoterpenes (1,8-cineole, linalool, camphor, borneol and bornyl acetate) as well as hydrocarbon (β-caryophyllene and α-humulene) and oxygenated sesquiterpenes (caryophyllene oxide, viridiflorol, humulene epoxide II and III) were found. The EO contained a significantly (p < 0.05) higher percentage of 1,8-cineole (10.4%), α-thujone (17.3%) and camphor (29.2%), whereas supercritical fluid (SF) extracts contained a significantly higher percentage of borneol (8.4%), bornyl acetate (2.2%), α-humulene (6.4%), viridiflorol (22.1%), humulene epoxide II and III (2.4% and 0.4%), and some unidentified sesquiterpene alcohols. Both EO and SF extracts contained equal amounts of β-thujone (4.8%) and β-caryophyllene (~7%). Our results show that HD of EO is a more efficient and economic method for α- and β-thujone extraction.

Comparative analysis of alpha- and beta-thujone in the essential oil and supercritical CO2 extract of sage (Salvia officinalis L.)

OCCHIPINTI, Andrea;CAPUZZO, ANDREA;MAFFEI, Massimo Emilio
2014

Abstract

Salvia officinalis L. (Sage) is an important industrial plant used both for food and pharmaceutical purposes. The terpene fraction of this plant, which contains thujones and other mono and sesquiterpenoids, is responsible for many of its therapeutic and culinary properties. We used two extraction methods [hydrodistillation (HD) to obtain the essential oil (EO), and supercritical CO2 extraction (SFE)] to analyze by gas chromatography–mass spectrometry (GC–MS) the terpene fraction extracted from sage dried leaves. α-Thujone, β-thujone and other oxygenated monoterpenes (1,8-cineole, linalool, camphor, borneol and bornyl acetate) as well as hydrocarbon (β-caryophyllene and α-humulene) and oxygenated sesquiterpenes (caryophyllene oxide, viridiflorol, humulene epoxide II and III) were found. The EO contained a significantly (p < 0.05) higher percentage of 1,8-cineole (10.4%), α-thujone (17.3%) and camphor (29.2%), whereas supercritical fluid (SF) extracts contained a significantly higher percentage of borneol (8.4%), bornyl acetate (2.2%), α-humulene (6.4%), viridiflorol (22.1%), humulene epoxide II and III (2.4% and 0.4%), and some unidentified sesquiterpene alcohols. Both EO and SF extracts contained equal amounts of β-thujone (4.8%) and β-caryophyllene (~7%). Our results show that HD of EO is a more efficient and economic method for α- and β-thujone extraction.
JOURNAL OF ESSENTIAL OIL RESEARCH
26
2
85
90
Salvia officinalis; Supercritical Fluid Etraction; Alpha and beta thujone
OCCHIPINTI A.; CAPUZZO A.; ARCEUSZ A.; MAFFEI M.
File in questo prodotto:
File Dimensione Formato  
Comparative_analysis_of_alpha_4aperto.pdf

accesso aperto

Descrizione: Articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 630.96 kB
Formato Adobe PDF
630.96 kB Adobe PDF Visualizza/Apri
2014 Jeor alfa beta Thujone.pdf

non disponibili

Descrizione: Articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 259.46 kB
Formato Adobe PDF
259.46 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/139585
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
social impact