Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor.

Quantitative analysis of herbivore-induced cytosolic calcium by using a Cameleon (YC 3.6) calcium sensor in Arabidopsis thaliana

OCCHIPINTI, Andrea;MAFFEI, Massimo Emilio
2014-01-01

Abstract

Ca(2+) is a key player in plant cell responses to biotic and abiotic stress. Owing to the central role of cytosolic Ca(2+) ([Ca(2+)]cyt) during early signaling and the need for precise determination of [Ca(2+)]cyt variations, we used a Cameleon YC 3.6 reporter protein expressed in Arabidopsis thaliana to quantify [Ca(2+)]cyt variations upon leaf mechanical damage (MD), herbivory by 3rd and 5th instar larvae of Spodoptera littoralis and S. littoralis oral secretions (OS) applied to MD. YC 3.6 allowed a clear distinction between MD and herbivory and discriminated between the two larvae instars. To our knowledge this is the first report of quantitative [Ca(2+)]cyt determination upon herbivory using a Cameleon calcium sensor.
2014
171
136
139
Arabidopsis thaliana; Calcium signaling; Cameleon YC 3.6; Herbivory; Spodoptera littoralis
VERRILLO F.; OCCHIPINTI A.; KANCHISWAMY C.N.; MAFFEI M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/139586
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