miR-21 is overexpressed in tumors and it displays oncogenic activity. Here, we show that expression of miR-21 in primary tumors anticorrelates with KRIT1/CCM1, an interacting partner of the Ras-like GTPase Rap1, involved in Cerebral Cavernous Malformations (CCM). We present evidences that miR-21 silences KRIT1 by targeting its mRNA 3'UTR and that this interaction is involved in tumor growth control. In fact, miR-21 over-expression or KRIT1 knock-down promote anchorage independent tumor cell growth compared to controls, whereas the opposite is observed when anti-miR-21 or KRIT1 overexpression are employed. Our findings suggest that miR-21 promotes tumor cell growth, at least in part, by down-modulating the potential tumor suppressor KRIT1.

miR-21 coordinates tumor growth and modulates KRIT1 levels

ORSO, FRANCESCA;BALZAC, Fiorella;MARINO, Marco;LEMBO, ANTONIO;RETTA, Saverio Francesco;TAVERNA, Daniela
2013

Abstract

miR-21 is overexpressed in tumors and it displays oncogenic activity. Here, we show that expression of miR-21 in primary tumors anticorrelates with KRIT1/CCM1, an interacting partner of the Ras-like GTPase Rap1, involved in Cerebral Cavernous Malformations (CCM). We present evidences that miR-21 silences KRIT1 by targeting its mRNA 3'UTR and that this interaction is involved in tumor growth control. In fact, miR-21 over-expression or KRIT1 knock-down promote anchorage independent tumor cell growth compared to controls, whereas the opposite is observed when anti-miR-21 or KRIT1 overexpression are employed. Our findings suggest that miR-21 promotes tumor cell growth, at least in part, by down-modulating the potential tumor suppressor KRIT1.
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http://www.sciencedirect.com/science/article/pii/S0006291X13011820
KRIT1; Cerebral Cavernous Malformations (CCM); miR-21; Tumor growth; Breast cancer; melanoma; Cancer; microRNA
F. Orso; F. Balzac; M. Marino; A. Lembo; S.F. Retta; D. Taverna
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/143014
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