Lactoperoxidase (LPO), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) belong to the class of haloperoxidase, a group of mammalian enzymes able to catalyze the peroxidative oxidation of halide and pseudohalide, like thiocyanate. They all play a key role in the development of antibacterial activity, whereas the homology in functional role is emphasized by the striking similarity of their primary structures. A theoretical model for the tridimensional structure of LPO and EPO has been elaborated on the basis of MPO X-ray structure, on account of the high degree of similarity found in their sequences. Some evidences supporting the hypothesis of an ester-linkage between heme and apoprotein in LPO and EPO, originally proposed by Hultquist & Morrison [J. Biol. Chem. (1963) 238, 2843-46], are discussed.

A theoretical three-dimensional model for lactoperoxidase and eosinophil peroxidase built on the scaffold of the myeloperoxidase X-ray structure

GHIBAUDI, Elena Maria;LAURENTI, Enzo;FERRARI, Rosa Pia
1996

Abstract

Lactoperoxidase (LPO), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) belong to the class of haloperoxidase, a group of mammalian enzymes able to catalyze the peroxidative oxidation of halide and pseudohalide, like thiocyanate. They all play a key role in the development of antibacterial activity, whereas the homology in functional role is emphasized by the striking similarity of their primary structures. A theoretical model for the tridimensional structure of LPO and EPO has been elaborated on the basis of MPO X-ray structure, on account of the high degree of similarity found in their sequences. Some evidences supporting the hypothesis of an ester-linkage between heme and apoprotein in LPO and EPO, originally proposed by Hultquist & Morrison [J. Biol. Chem. (1963) 238, 2843-46], are discussed.
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Lactoperoxidase; Eosinophil peroxidase; Myeloperoxidase; Molecular Modelling; Protein structure
DE GIOIA L.; GHIBAUDI E.M.; LAURENTI E.; SALMONA M.; R. FERRARI
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1485
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