We applied a direct competitive immunoassay for measuring corticosterone and glucocorticoid metabolites in faeces (FGMs) as a non-invasive tool for monitoring the stress response of African penguins (Spheniscus demersus) held in captivity in a zoological facility. The developed assay, validated in-house, proved to be rapid (the test could be completed in 90 minutes), sensitive (LOD for corticosterone 0.0002 mg/l, dynamic range 0.00075–0.075 mg/l) and broad-selective, as it cross-reacted with the major corticosteroids, thus allowing the detection of excreted FGMs resulting from a biological stressor. Matrix interference, due to components of faecal samples, was overcome by diluting sample extracts (1 + 4 or 1 + 9, depending on the sample). The assay enabled us to investigate the response to stress in five animals – three adult males and two adult females – over a period of 30 hours, and to identify the peak of FGM production as being 7–10 hours after the stressful event

A broad-selective enzyme immunoassay for non-invasive stress assessment in African Penguins 1 (Spheniscus demersus) held in captivity

ANFOSSI, Laura;OZELLA, Laura;DI NARDO, FABIO;GIOVANNOLI, Cristina;PASSINI, CINZIA;FAVARO, LIVIO;PESSANI, Daniela;BAGGIANI, Claudio
2014

Abstract

We applied a direct competitive immunoassay for measuring corticosterone and glucocorticoid metabolites in faeces (FGMs) as a non-invasive tool for monitoring the stress response of African penguins (Spheniscus demersus) held in captivity in a zoological facility. The developed assay, validated in-house, proved to be rapid (the test could be completed in 90 minutes), sensitive (LOD for corticosterone 0.0002 mg/l, dynamic range 0.00075–0.075 mg/l) and broad-selective, as it cross-reacted with the major corticosteroids, thus allowing the detection of excreted FGMs resulting from a biological stressor. Matrix interference, due to components of faecal samples, was overcome by diluting sample extracts (1 + 4 or 1 + 9, depending on the sample). The assay enabled us to investigate the response to stress in five animals – three adult males and two adult females – over a period of 30 hours, and to identify the peak of FGM production as being 7–10 hours after the stressful event
ANALYTICAL METHODS
6
8222
8231
L.Anfossi; L.Ozella; F.Di Nardo; C.Giovannoli; C.Passini; L.Favaro; D.Pessani; E.Mostl; C.Baggiani
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/149441
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