An association study between the milk yield trait and the Stearoyl-CoA Desaturase (SCD) polymorphism (g.133A>C) in Italian Mediterranean river buffalo was carried out. A full characterization of the river buffalo SCD promoter region was presented. Genotyping information was provided and a quick method for allelic discrimination was developed. The frequency of the C allele was 0•16. Test day (TD) records (43,510) of milk production belonging to 226 lactations of 169 buffalo cows were analyzed with a mixed linear model in order to estimate the effect of g.133A>C genotype, as well as the effect of parity and calving season. The SCD genotype was significantly associated with milk yield (P = 0•02). The genotype AC showed an over-dominance effect with an average daily milk yield approximately 2 kg/d higher than CC buffaloes. Such a difference represents about 28% more milk per day. The effect of the genotype was constant across lactation stages. The contribution of SCD genotype (r2SCD) to the total phenotypic variance in milk yield was equal to 0•12. This report is among the first indications of genetic association between a trait of economic importance in river buffalo. Although such results need to be confirmed with large-scale studies in the same and other buffalo populations, they might offer useful indications for the application of MAS programs in river buffalo and in the future they might be of great economic interest for the river buffalo dairy industry.

A single nucleotide polymorphism (SNP) in the promoter region of River buffalo Stearoyl CoA Desaturase gene (SCD) is associated with milk yield

PAUCIULLO, Alfredo;
2012-01-01

Abstract

An association study between the milk yield trait and the Stearoyl-CoA Desaturase (SCD) polymorphism (g.133A>C) in Italian Mediterranean river buffalo was carried out. A full characterization of the river buffalo SCD promoter region was presented. Genotyping information was provided and a quick method for allelic discrimination was developed. The frequency of the C allele was 0•16. Test day (TD) records (43,510) of milk production belonging to 226 lactations of 169 buffalo cows were analyzed with a mixed linear model in order to estimate the effect of g.133A>C genotype, as well as the effect of parity and calving season. The SCD genotype was significantly associated with milk yield (P = 0•02). The genotype AC showed an over-dominance effect with an average daily milk yield approximately 2 kg/d higher than CC buffaloes. Such a difference represents about 28% more milk per day. The effect of the genotype was constant across lactation stages. The contribution of SCD genotype (r2SCD) to the total phenotypic variance in milk yield was equal to 0•12. This report is among the first indications of genetic association between a trait of economic importance in river buffalo. Although such results need to be confirmed with large-scale studies in the same and other buffalo populations, they might offer useful indications for the application of MAS programs in river buffalo and in the future they might be of great economic interest for the river buffalo dairy industry.
2012
79
4
429
435
Mediterranean river buffalo; stearoyl CoA desaturase gene (SCD; marker assisted selection; Milk yield
PAUCIULLO A.; COSENZA G.; STERI R.; COLETTA A.; LA BATTAGLIA A.; DI BERARDINO D.; MACCIOTTA N. N. P.; RAMUNNO L.
File in questo prodotto:
File Dimensione Formato  
Pauciullo et al. SCD ugov.pdf

Accesso aperto

Tipo di file: PDF EDITORIALE
Dimensione 201.52 kB
Formato Adobe PDF
201.52 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/151376
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 23
social impact