Abstract Introduction: PTEN plays an essential role in the pathogenesis of chronic myeloid leukemia. Recently, we have shown that BCR-ABL promotes PTEN nuclear exclusion, through the modulation of HAUSP activity. Objectives: Here, we investigate HAUSP cellular compartmentalization in primary CML samples. Results: While in normal CD34 positive cells HAUSP is expressed mostly in the nucleus, in CML CD34 cells HAUSP is expressed both in the nuclear bodies and in the cytoplasm. Conclusions: This observation suggests that HAUSP behaves as a shuttling protein in CML. It can bind to BCR-ABL in the cytosol, where it is phosphorylated on tyrosine residues, and it maintains the proper compartmentalization in the nuclear bodies, where it acts as part of a PML network to regulate PTEN de-ubiquitination.

HAUSP compartmentalization in chronic myeloid leukemia

MOROTTI, Alessandro;PANUZZO, Cristina;CRIVELLARO, SABRINA;CARRA', GIOVANNA;GUERRASIO, Angelo;SAGLIO, Giuseppe
2014

Abstract

Abstract Introduction: PTEN plays an essential role in the pathogenesis of chronic myeloid leukemia. Recently, we have shown that BCR-ABL promotes PTEN nuclear exclusion, through the modulation of HAUSP activity. Objectives: Here, we investigate HAUSP cellular compartmentalization in primary CML samples. Results: While in normal CD34 positive cells HAUSP is expressed mostly in the nucleus, in CML CD34 cells HAUSP is expressed both in the nuclear bodies and in the cytoplasm. Conclusions: This observation suggests that HAUSP behaves as a shuttling protein in CML. It can bind to BCR-ABL in the cytosol, where it is phosphorylated on tyrosine residues, and it maintains the proper compartmentalization in the nuclear bodies, where it acts as part of a PML network to regulate PTEN de-ubiquitination.
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Alessandro Morotti*; Cristina Panuzzo*; Sabrina Crivellaro; Giovanna Carra; Angelo Guerrasio; Giuseppe Saglio
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/151478
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