The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.

A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.

REHMAN, Michael;
2010-01-01

Abstract

The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of approximately 400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2, a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.
2010
17
901
908
http://dx.doi.org/10.1038/nsmb.1829
Cell Membrane; genetics/metabolism, Endocytosis, Endosomes; genetics/metabolism, Genes; Fungal, Membrane Proteins; genetics/metabolism, Saccharomyces cerevisiae Proteins; genetics/metabolism, Saccharomyces cerevisiae; genetics/metabolism, Sphingolipids; genetics/metabolism
P. S. Aguilar;F. Fröhlich;M. Rehman;M. Shales;I. Ulitsky;A. Olivera-Couto;H. Braberg;R. Shamir;P. Walter;M. Mann;C. S. Ejsing;N. J. Krogan;T. C. Walther
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/152179
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