The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) – a single isomer, cationic CD derivative – developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs.

Cationic permethylated 6-monoamino-6-monodeoxy--cyclodextrin as chiral selector of dansylated amino acids in capillary electrophoresis

JICSINSZKY, Laszlo;
2014-01-01

Abstract

The resolution power of permethylated 6-monoamino-6-monodeoxy-βCD (PMMABCD) – a single isomer, cationic CD derivative – developed previously for chiral analyses in capillary electrophoresis was further studied here. Dansylated amino acids (Dns-AA) were chosen as amphoteric chiral model compounds. Changes in the resolutions of Dns-AAs by varying pH and selector concentrations were investigated and correlated with their structures and chemical properties (isoelectric point and lipophilicity). Maximal resolutions could be achieved at pH 6 or pH 4. The separations improved with increasing concentration of the selector. Baseline or substantially better resolution for 8 pairs of these Dns-AAs could be achieved. Low CD concentration was enough for the separation of the most apolar Dns-AAs. Chiral discrimination ability of PMMABCD was demonstrated by the separation of an artificial mixture of 8 Dns-AA pairs.
2014
99
16
21
http://www.sciencedirect.com/science/article/pii/S0731708514003161
Krisztina Nemeth; Celesztina Domonkos; Virag Sarnyai; Julianna Szeman; Laszlo Jicsinszky; Lajos Szente; Julia Visy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/154824
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