Background.This study investigated whether the 1 : 2 -3/-6 ratio may reduce proinflammatory response in human alveolar cells (A549) exposed to an ex vivo inflammatory stimulus (bronchoalveolar lavage fluid (BALF) of acute respiratory distress syndrome (ARDS) patients). Methods. We exposed A549 cells to the BALF collected from 12 ARDS patients. After 18 hours, fatty acids (FA) were added as docosahexaenoic acid (DHA, -3) and arachidonic acid (AA, -6) in two ratios (1 : 2 or 1 : 7). 24 hours later, in culture supernatants were evaluated cytokines (TNF-, IL-6, IL-8, and IL-10) and prostaglandins (PGE2 and PGE3) release. The FA percentage content in A549 membrane phospholipids, content of COX-2, level of PPAR, and NF-B binding activity were determined. Results.The 1 : 2 DHA/AA ratio reversed the baseline predominance of -6 over -3 in the cellmembranes (P < 0.001). Theproinflammatory cytokine release was reduced by the 1 : 2 ratio (P < 0.01 to <0.001) but was increased by the 1 : 7 ratio (P < 0.01). The 1 : 2 ratio reduced COX-2 and PGE2 (P < 0.001) as well as NF-B translocation into the nucleus (P < 0.01), while it increased activation of PPAR and IL-10 release (P < 0.001). Conclusion.This study demonstrated that shifting the FA supply from -6 to -3 decreased proinflammatory mediator release in human alveolar cells exposed to BALF of ARDS patients.

The Omega-3 fatty acid docosahexaenoic acid modulates inflammatory mediator release in human alveolar cells exposed to bronchoalveolar lavage fluid of ards patients

COTOGNI, PAOLO;TROMBETTA, Antonella;MUZIO, Giuliana;MAGGIORA, Marina;CANUTO, Rosa Angela
2015-01-01

Abstract

Background.This study investigated whether the 1 : 2 -3/-6 ratio may reduce proinflammatory response in human alveolar cells (A549) exposed to an ex vivo inflammatory stimulus (bronchoalveolar lavage fluid (BALF) of acute respiratory distress syndrome (ARDS) patients). Methods. We exposed A549 cells to the BALF collected from 12 ARDS patients. After 18 hours, fatty acids (FA) were added as docosahexaenoic acid (DHA, -3) and arachidonic acid (AA, -6) in two ratios (1 : 2 or 1 : 7). 24 hours later, in culture supernatants were evaluated cytokines (TNF-, IL-6, IL-8, and IL-10) and prostaglandins (PGE2 and PGE3) release. The FA percentage content in A549 membrane phospholipids, content of COX-2, level of PPAR, and NF-B binding activity were determined. Results.The 1 : 2 DHA/AA ratio reversed the baseline predominance of -6 over -3 in the cellmembranes (P < 0.001). Theproinflammatory cytokine release was reduced by the 1 : 2 ratio (P < 0.01 to <0.001) but was increased by the 1 : 7 ratio (P < 0.01). The 1 : 2 ratio reduced COX-2 and PGE2 (P < 0.001) as well as NF-B translocation into the nucleus (P < 0.01), while it increased activation of PPAR and IL-10 release (P < 0.001). Conclusion.This study demonstrated that shifting the FA supply from -6 to -3 decreased proinflammatory mediator release in human alveolar cells exposed to BALF of ARDS patients.
2015
2015
642520
642530
http://www.hindawi.com/journals/biomed/
Arachidonic Acid; Bronchoalveolar Lavage Fluid; Cyclooxygenase 2; Dinoprostone; Docosahexaenoic Acids; Fatty Acids, Omega-3; Fatty Acids, Omega-6; Gene Expression Regulation; Humans; Inflammation; Inflammation Mediators; Interleukin-10; NF-kappa B; PPAR gamma; Pulmonary Alveoli; Respiratory Distress Syndrome, Adult; Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all)
Cotogni, Paolo; Trombetta, Antonella; Muzio, Giuliana; Maggiora, Marina; Canuto, Rosa Angela
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1564424
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