The most common translocation in chronic myeloid leukemia (CML) t(9;22) (q34;q22) produces the BCR/ABL fusion gene. We set up and evaluated a rapid and reliable real-time reverse-transcription-polymerase chain reaction (RT-PCR) approach using TaqMan technology for detection and quantification of bcr-abl transcripts in CML patients at diagnosis and during therapy.

Real-time quantification of different types of bcr-abl transcript in chronic myeloid leukemia

SAGLIO, Giuseppe;
2001-01-01

Abstract

The most common translocation in chronic myeloid leukemia (CML) t(9;22) (q34;q22) produces the BCR/ABL fusion gene. We set up and evaluated a rapid and reliable real-time reverse-transcription-polymerase chain reaction (RT-PCR) approach using TaqMan technology for detection and quantification of bcr-abl transcripts in CML patients at diagnosis and during therapy.
2001
86
3
252
259
Bone Marrow; Female; Genes, abl; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Male; Methods; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
Amabile, M; Giannini, B; Testoni, N; Montefusco, V; Rosti, G; Zardini, C; Terragna, C; Buonamici, S; Ottaviani, E; Soverini, S; Fiacchini, M; Bassi, S...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1592643
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