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EnglishA novel and disposable cartridge for chemiluminescent (CL)-lateral flow immunoassay (LFIA) with integrated amorphous silicon (a-Si:H) photosensors array was developed and applied to quantitatively detect human serum albumin (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horseradish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors deposited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable read-out electronics. The method is simple and fast with a detection limit of 2.5 mug L−1 for HSA in urine and a dynamic range up to 850 mug L−1, which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro- and macroalbuminuria). The use of CL detection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to picomoles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides compact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses
| Titolo: | Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples | |
| Autori Riconosciuti: | ||
| Autori: | Martina Zangheri; Fabio Di Nardo; Mara Mirasoli; Laura Anfossi; Augusto Nascetti; Domenico Caputo; Giampiero De Cesare; Massimo Guardigli; Claudio Baggiani; Aldo Roda | |
| Data di pubblicazione: | 2016 | |
| Abstract: | A novel and disposable cartridge for chemiluminescent (CL)-lateral flow immunoassay (LFIA) with integrated amorphous silicon (a-Si:H) photosensors array was developed and applied to quantitatively detect human serum albumin (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horseradish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors deposited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable read-out electronics. The method is simple and fast with a detection limit of 2.5 mug L−1 for HSA in urine and a dynamic range up to 850 mug L−1, which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro- and macroalbuminuria). The use of CL detection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to picomoles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides compact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses | |
| Volume: | 408 | |
| Pagina iniziale: | 8869 | |
| Pagina finale: | 8879 | |
| Digital Object Identifier (DOI): | 10.1007/s00216-016-9991-0 | |
| Rivista: | ANALYTICAL AND BIOANALYTICAL CHEMISTRY | |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| abc16_408_8869_draft.pdf | preprint | PREPRINT (PRIMA BOZZA) | Open Access Visualizza/Apri | |
| abc16_408_8869.pdf | pdf editoriale | PDF EDITORIALE | Utenti riconosciuti Richiedi una copia | |
| abc16_408_8869_si.pdf | supporting informations | MATERIALE NON BIBLIOGRAFICO | Open Access Visualizza/Apri |
http://hdl.handle.net/2318/1632653
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