The electroantennogram responses (EAGs) of Macrolophus melanotoma and Macrolophus pygmaeus (Hemiptera: Miridae) exposed to volatile compounds (VOCs) of host and non-host plants were compared. The VOCs were identified by gas chromatography. Hosts and non-hosts eliciting similar EAGs were tested in olfactory assays against plants without a significant EAGs for the two Macrolophus species. No characteristic VOC profile was found for hosts and non-hosts. Terpenes predominated in many hosts and carboxylic acids in non-hosts, but no specific VOCs were characteristic of host plants. Significant EAGs (maximum deflection values in mV) were recorded in plants with very different VOC profiles, both hosts and nonhosts. The EAGs were higher for M. melanotoma than for M. pygmaeus, and were higher for males than for females. In M. melanotoma the EAGs were greater with hosts than with non-hosts, but they were similar in M. pygmaeus. The EAGs were correlated with the concentrations of sesquiterpenes and alcohols in both species. In olfactory assays, M. melanotoma and M. pygmaeus preferred their respective hosts, but they did not discriminate between non-host with and without significant EAGs. According to the results, Macrolophus species are expected to rely on ubiquitous VOCs for the identification of their hosts. The variation in the EAGs between M. melanotoma and M. pygmaeus is attributed to the variation in the proportions of olfactory receptor neurones with different sensitivity to VOCs (e.g. sesquiterpenes). Host plant selection is discussed in the light of the perception of VOCs and the processing of information by the central nervous system.

Host plant perception and selection in the sibling species Macrolophus melanotoma and Macrolophus pygmaeus (Hemiptera: Miridae)

Ingegno Bl;Tavella L;
2016

Abstract

The electroantennogram responses (EAGs) of Macrolophus melanotoma and Macrolophus pygmaeus (Hemiptera: Miridae) exposed to volatile compounds (VOCs) of host and non-host plants were compared. The VOCs were identified by gas chromatography. Hosts and non-hosts eliciting similar EAGs were tested in olfactory assays against plants without a significant EAGs for the two Macrolophus species. No characteristic VOC profile was found for hosts and non-hosts. Terpenes predominated in many hosts and carboxylic acids in non-hosts, but no specific VOCs were characteristic of host plants. Significant EAGs (maximum deflection values in mV) were recorded in plants with very different VOC profiles, both hosts and nonhosts. The EAGs were higher for M. melanotoma than for M. pygmaeus, and were higher for males than for females. In M. melanotoma the EAGs were greater with hosts than with non-hosts, but they were similar in M. pygmaeus. The EAGs were correlated with the concentrations of sesquiterpenes and alcohols in both species. In olfactory assays, M. melanotoma and M. pygmaeus preferred their respective hosts, but they did not discriminate between non-host with and without significant EAGs. According to the results, Macrolophus species are expected to rely on ubiquitous VOCs for the identification of their hosts. The variation in the EAGs between M. melanotoma and M. pygmaeus is attributed to the variation in the proportions of olfactory receptor neurones with different sensitivity to VOCs (e.g. sesquiterpenes). Host plant selection is discussed in the light of the perception of VOCs and the processing of information by the central nervous system.
29
2
117
142
https://link.springer.com/article/10.1007/s10905-016-9549-1
Electroantennography (EAG), olfactometry, gas chromatography (GC), mass spectrometry (MS), generalist predators, volatile organic compounds (VOCs), host plants, plant choice, peripheral olfactory system
Ingegno, BARBARA LETIZIA; La-spina, M; Jordan, Mj; Tavella, Luciana; Sanchez, Ja
File in questo prodotto:
File Dimensione Formato  
Ingegno_et_al_2016_Host Plant Perception and Selection in the Sibling Species.pdf

Accesso riservato

Descrizione: Articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 659.96 kB
Formato Adobe PDF
659.96 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Ingegno_JInsectBehavior2016_postprint.pdf

Accesso aperto con embargo fino al 19/02/2017

Descrizione: Articolo principale
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 489.93 kB
Formato Adobe PDF
489.93 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1652699
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact