A lateral flow immunoassay (LFIA) was developed for the determination of fumonisin mycotoxins. CdSe/ZnS quantum dots (QDs) fluorescence, observed at excitation/emission wavelengths of 365/525 nm, is suppressed by the addition of silver nanoparticles (SNPs) or gold nanoparticles (GNPs) because SNPs overlap the excitation bands of the QDs, and GNPs overlap the emission bands. The fluorescence of the QDs is recovered upon addition of fumonisins, allowing for the sensitive detection in "positive mode" of the target mycotoxin by monitoring the changes of the QDs fluorescence intensity. The SNPs are found to be the most effective quenchers, while the use of GNPs allows for an efficient recovery of fluorescence and can be employed in the LFIA. The method was successfully applied to the fluorometric determination of fumonisins in spiked maize flour samples. The visual detection limit is at the ng⋅mL¹ level. This is four-times lower compared to the colorimetric LFIA based on the use of the traditional gold NPs

A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles

Anfossi, Laura;Di Nardo, Fabio;CAVALERA, SIMONE;Giovannoli, Cristina;Spano, Giulia;Baggiani, Claudio
2018

Abstract

A lateral flow immunoassay (LFIA) was developed for the determination of fumonisin mycotoxins. CdSe/ZnS quantum dots (QDs) fluorescence, observed at excitation/emission wavelengths of 365/525 nm, is suppressed by the addition of silver nanoparticles (SNPs) or gold nanoparticles (GNPs) because SNPs overlap the excitation bands of the QDs, and GNPs overlap the emission bands. The fluorescence of the QDs is recovered upon addition of fumonisins, allowing for the sensitive detection in "positive mode" of the target mycotoxin by monitoring the changes of the QDs fluorescence intensity. The SNPs are found to be the most effective quenchers, while the use of GNPs allows for an efficient recovery of fluorescence and can be employed in the LFIA. The method was successfully applied to the fluorometric determination of fumonisins in spiked maize flour samples. The visual detection limit is at the ng⋅mL¹ level. This is four-times lower compared to the colorimetric LFIA based on the use of the traditional gold NPs
MIKROCHIMICA ACTA
185
2
94
103
http://www.springer/at/mca
FRET; Gold nanoparticles; Immunochromatography; Inner filter effect; Silver nanoparticles; Strip test; Analytical Chemistry
Anfossi, Laura*; Di Nardo, Fabio; Cavalera, Simone; Giovannoli, Cristina; Spano, Giulia; Speranskaya, Elena S.; Goryacheva, Irina Y.; Baggiani, Claudio
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1660432
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