The study of the so-called unculturable bacteria is still considered a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, the identification and characterization of such microbes in complex biological samples is now possible. In this chapter we report how endobacteria thriving inside arbuscular mycorrhizal fungi (AMF), which are themselves obligate biotrophs of plants, can be studied using a combination of in vitro culture, molecular biology, and microscopy techniques.

Investigating the endobacteria which thrive in arbuscular mycorrhizal fungi

Salvioli, Alessandra;Bonfante, Paola
2016-01-01

Abstract

The study of the so-called unculturable bacteria is still considered a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, the identification and characterization of such microbes in complex biological samples is now possible. In this chapter we report how endobacteria thriving inside arbuscular mycorrhizal fungi (AMF), which are themselves obligate biotrophs of plants, can be studied using a combination of in vitro culture, molecular biology, and microscopy techniques.
2016
Methods in Molecular Biology
Humana Press Inc.
1399
29
53
978-1-4939-3367-9
978-1-4939-3369-3
http://www.springer.com/series/7651
Arbuscular mycorrhizal fungi; Bacterial enrichment; Candidatus Glomeribacter gigasporarum; Endobacteria; Fluorescent in situ hybridization; Mollicutes-related endobacteria; Real-time quantitative PCR; Transmission electron microscopy; Bacteria; Genome, Bacterial; Mycorrhizae; Phylogeny; Plants; RNA, Ribosomal, 16S; Spores, Fungal; Symbiosis; Molecular Biology; Genetics
Desirò, Alessandro; Salvioli, Alessandra; Bonfante, Paola
File in questo prodotto:
File Dimensione Formato  
Desirò et al., 2016.pdf

Accesso aperto

Descrizione: postprint Desirò et al., 2016
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 828.77 kB
Formato Adobe PDF
828.77 kB Adobe PDF Visualizza/Apri
318406_Microbial Environmental Genomics.pdf

Accesso riservato

Descrizione: PDF editoriale
Tipo di file: PDF EDITORIALE
Dimensione 6.09 MB
Formato Adobe PDF
6.09 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1660487
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 8
  • ???jsp.display-item.citation.isi??? ND
social impact