Background: Activation of free fatty acid receptor 2 (FFAR2) by microbiota-derived metabolites (e.g., propionate) reduces leukaemic cell proliferation in vitro. This study aims to test whether Ffar2 expression per se also influences leukaemia cell growth in vivo. Methods: Bcr-Abl-expressing BaF cells were used as a leukaemia model and the role of Ffar2 was evaluated in Balb/c mice after lentiviral shRNA transduction. Results: Our data formally establish that reduced leukaemic cell proliferation is associated with increased Ffar2 expression in vivo and in vitro. Going beyond association, we point out that decreasing Ffar2 expression fosters cancer cell growth in vitro and in vivo. Conclusions: Our data demonstrate the role of Ffar2 in the control of leukaemic cell proliferation in vivo and indicate that a modulation of Ffar2 expression through nutritional tools or pharmacological agents may constitute an attractive therapeutic approach to tackle leukaemia progression in humans.

Ffar2 expression regulates leukaemic cell growth in vivo

Porporato, Paolo E.;
2017

Abstract

Background: Activation of free fatty acid receptor 2 (FFAR2) by microbiota-derived metabolites (e.g., propionate) reduces leukaemic cell proliferation in vitro. This study aims to test whether Ffar2 expression per se also influences leukaemia cell growth in vivo. Methods: Bcr-Abl-expressing BaF cells were used as a leukaemia model and the role of Ffar2 was evaluated in Balb/c mice after lentiviral shRNA transduction. Results: Our data formally establish that reduced leukaemic cell proliferation is associated with increased Ffar2 expression in vivo and in vitro. Going beyond association, we point out that decreasing Ffar2 expression fosters cancer cell growth in vitro and in vivo. Conclusions: Our data demonstrate the role of Ffar2 in the control of leukaemic cell proliferation in vivo and indicate that a modulation of Ffar2 expression through nutritional tools or pharmacological agents may constitute an attractive therapeutic approach to tackle leukaemia progression in humans.
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http://www.nature.com/bjc/index.html
cell proliferation; CMTB; free fatty acid receptor; FFA2; GPR43; leukaemic cells; propionate; short-chain fatty acids; Animals; Apoptosis; Biomarkers, Tumor; Female; Leukemia, Experimental; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Receptors, G-Protein-Coupled; Tumor Cells, Cultured; Cell Proliferation; Oncology; Cancer Research
Bindels, Laure B.; Porporato, Paolo E.; Ducastel, Sarah; Sboarina, Martina; Neyrinck, Audrey M.; Dewulf, Evelyne M.; Feron, Olivier; Lestavel, Sophie; Cani, Patrice D.; Staels, Bart; Sonveaux, Pierre; Delzenne, Nathalie M.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1661703
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