A few compounds in which the nitric oxide (NO) photodonor N-[4-nitro-3-(trifluoromethyl)phenyl]propane-1,3-diamine is joined to the mitochondria-targeting alkyltriphenylphosphonium moiety via flexible spacers of variable length were synthesized. The lipophilicity of the products was evaluated by measuring their partition coefficients in n-octanol/water. The obtained values, markedly lower than those calculated, are consistent with the likely collapsed conformation assumed by the compounds in solution, as suggested by molecular dynamics simulations. The capacity of the compounds to release NO under visible light irradiation was evaluated by measuring nitrite production by means of the Griess reaction. The accumulation of compounds in the mitochondria of human lung adenocarcinoma A549 cells was assessed by UPLC-MS. Interestingly, compound 13 [(9-((3-((4-nitro-3-(trifluoromethyl)phenyl)amino)propyl)amino)-9-oxononyl) triphenylphosphonium bromide] displayed both the highest accumulation value and high toxicity toward A549 cells upon irradiation-mediated NO release in mitochondria.

Tuning the Hydrophobicity of a Mitochondria-Targeted NO Photodonor

Sodano, Federica;Rolando, Barbara;Spyrakis, Francesca;FAILLA, Mariacristina;Lazzarato, Loretta;Gazzano, Elena;Riganti, Chiara;Fruttero, Roberta;Gasco, Alberto;Sortino, Salvatore
2018

Abstract

A few compounds in which the nitric oxide (NO) photodonor N-[4-nitro-3-(trifluoromethyl)phenyl]propane-1,3-diamine is joined to the mitochondria-targeting alkyltriphenylphosphonium moiety via flexible spacers of variable length were synthesized. The lipophilicity of the products was evaluated by measuring their partition coefficients in n-octanol/water. The obtained values, markedly lower than those calculated, are consistent with the likely collapsed conformation assumed by the compounds in solution, as suggested by molecular dynamics simulations. The capacity of the compounds to release NO under visible light irradiation was evaluated by measuring nitrite production by means of the Griess reaction. The accumulation of compounds in the mitochondria of human lung adenocarcinoma A549 cells was assessed by UPLC-MS. Interestingly, compound 13 [(9-((3-((4-nitro-3-(trifluoromethyl)phenyl)amino)propyl)amino)-9-oxononyl) triphenylphosphonium bromide] displayed both the highest accumulation value and high toxicity toward A549 cells upon irradiation-mediated NO release in mitochondria.
CHEMMEDCHEM
13
12
1238
1245
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1860-7187
Mitochondria; Nitric oxide; Phosphonium salts; Photoactivation; Targeted therapy; Molecular Medicine; Pharmacology, Toxicology and Pharmaceutics (all); Organic Chemistry
Sodano, Federica; Rolando, Barbara; Spyrakis, Francesca; Failla, Mariacristina; Lazzarato, Loretta*; Gazzano, Elena; Riganti, Chiara; Fruttero, Roberta; Gasco, Alberto; Sortino, Salvatore
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1670019
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