Visceral leishmaniasis (VL) is a zoonotic infectious disease with a severe impact on humans and animals. Infection is transmitted by phlebotomine sand flies, and several domestic and wild mammals act as reservoirs for the infection, so the prompt detection of infected hosts is crucial to preventing and controlling the spread of the disease and its transmission to humans. A rapid and portable tool for VL diagnosis based on the lateral flow immunoassay (LFIA) technology is described herein. The device exploits a highly specific chimeric recombinant antigen as the recognition element for capturing anti-leishmanial antibodies, and protein A labelled with gold nanoparticles as the signal reporter. The LFIA shows excellent diagnostic sensitivity (98.4%), specificity (98.9%), and agreement with serological reference methods for diagnosing canine VL. The long-termstability of the LFIA device was confirmed based on six months of storage at room temperature or 4 °C, and the qualitative response of the device was not affected by limited thermal stress. The use of the broadly specific protein A means that the LFIA can be readily adapted to diagnose VL in dogs (the main reservoir for human infection) and other mammals, thus further assisting efforts to control the spread of VL

A versatile and sensitive lateral flow immunoassay for the rapid diagnosis of visceral leishmaniasis

Anfossi, Laura;Di Nardo, Fabio;Profiti, Margherita;Nogarol, Chiara;Cavalera, Simone;Baggiani, Claudio;Giovannoli, Cristina;Spano, Giulia;Ferroglio, Ezio;Rosati, Sergio
2018-01-01

Abstract

Visceral leishmaniasis (VL) is a zoonotic infectious disease with a severe impact on humans and animals. Infection is transmitted by phlebotomine sand flies, and several domestic and wild mammals act as reservoirs for the infection, so the prompt detection of infected hosts is crucial to preventing and controlling the spread of the disease and its transmission to humans. A rapid and portable tool for VL diagnosis based on the lateral flow immunoassay (LFIA) technology is described herein. The device exploits a highly specific chimeric recombinant antigen as the recognition element for capturing anti-leishmanial antibodies, and protein A labelled with gold nanoparticles as the signal reporter. The LFIA shows excellent diagnostic sensitivity (98.4%), specificity (98.9%), and agreement with serological reference methods for diagnosing canine VL. The long-termstability of the LFIA device was confirmed based on six months of storage at room temperature or 4 °C, and the qualitative response of the device was not affected by limited thermal stress. The use of the broadly specific protein A means that the LFIA can be readily adapted to diagnose VL in dogs (the main reservoir for human infection) and other mammals, thus further assisting efforts to control the spread of VL
2018
410
17
4123
4134
link.springer.de/link/service/journals/00216/index.htm
Cat; Fox; Immunochromatographic test; Point-of-care test; Shelf-life; Validation; Analytical Chemistry; Biochemistry
Anfossi, Laura*; Di Nardo, Fabio; Profiti, Margherita; Nogarol, Chiara; Cavalera, Simone; Baggiani, Claudio; Giovannoli, Cristina; Spano, Giulia; Ferroglio, Ezio; Mignone, Walter; Rosati, Sergio
File in questo prodotto:
File Dimensione Formato  
abc18_410_4123.pdf

Accesso riservato

Descrizione: versione finale
Tipo di file: PDF EDITORIALE
Dimensione 1.37 MB
Formato Adobe PDF
1.37 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
abc18_410_4123draft.pdf

Accesso aperto

Descrizione: preprint
Tipo di file: PREPRINT (PRIMA BOZZA)
Dimensione 1.01 MB
Formato Adobe PDF
1.01 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1671270
Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 34
social impact