Prosthetic joint infection diagnosis is often difficult since biofilm-embedded microorganisms attach well to the prosthetic surfaces and resist their detection by conventional methods. DL-dithiothreitol has been described as a valid method for biofilm detachment on orthopedic devices. We report the case of an occasional detection of Listeria monocytogenes in a non immuno-compromised patient with a preoperative diagnosis of aseptic loosening. The infection diagnosis due to such rare bacteria was made postoperatively, thanks to a DL-dithiothreitol-based device. This may be considered a feasible approach for the microbiological analysis of prosthetic joint infection, considering that a prompt diagnosis of such biofilmassociated infections could bring some advantages, such as an early and appropriate antibiotic therapy administration and a reduction of undiagnosed infections.

Unexpected Listeria monocytogenes detection with a dithiothreitol-based device during an aseptic hip revision [*G. Banche and A. Bistolfi contributed equally to this work; ** V. Allizond is the corresponding author]

G. Banche;V. Allizond;C. Merlino;A. Massè;A. M. Cuffini
2018

Abstract

Prosthetic joint infection diagnosis is often difficult since biofilm-embedded microorganisms attach well to the prosthetic surfaces and resist their detection by conventional methods. DL-dithiothreitol has been described as a valid method for biofilm detachment on orthopedic devices. We report the case of an occasional detection of Listeria monocytogenes in a non immuno-compromised patient with a preoperative diagnosis of aseptic loosening. The infection diagnosis due to such rare bacteria was made postoperatively, thanks to a DL-dithiothreitol-based device. This may be considered a feasible approach for the microbiological analysis of prosthetic joint infection, considering that a prompt diagnosis of such biofilmassociated infections could bring some advantages, such as an early and appropriate antibiotic therapy administration and a reduction of undiagnosed infections.
FUTURE MICROBIOLOGY
13
1295
1299
DL-dithiothreitol, Listeria monocytogenes , prosthetic joint infections
G. Banche*, A. Bistolfi*, V. Allizond**, C. Galletta, M.R. Iannantuoni, E.S. Marra, C. Merlino, A. Massè, A.M. Cuffini
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1678111
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