Nowadays, the treatment of hypertension represents an important issue, particularly in developed countries. While in most cases the standard therapeutic approaches, consisting in the administration of 1 to 3 drugs, are adequate to reach adequate blood pressure levels, in some cases more drugs are needed: this condition is called "resistant hypertension". In this context, the administration of a diuretic, such as spironolactone or canrenoate salts, represents a standard practice. Since a reliable discrimination of real cases of resistant hypertension from cases of poor therapeutic adherence is currently difficult to obtain, the adoption of therapeutic drug monitoring has been suggested as a useful tool for this purpose. In this work, the authors developed and validated a simple, cheap and fast dilute-and-shot method with UHPLC-PDA analysis for the quantification of spironolactone and its metabolite canrenone in human urine samples.

A simple UHPLC-PDA method with a fast dilute-and-shot sample preparation for the quantification of canrenone and its prodrug spironolactone in human urine samples

Avataneo, Valeria
Co-first
;
De Nicolò, Amedeo
Co-first
;
Sciandra, Mauro;Tosello, Francesco;Cusato, Jessica;Perlo, Elisa;Fatiguso, Giovanna;Allegra, Sarah;Favata, Fabio;Mulatero, Paolo;Veglio, Franco;Di Perri, Giovanni;D'Avolio, Antonio
Last
2018

Abstract

Nowadays, the treatment of hypertension represents an important issue, particularly in developed countries. While in most cases the standard therapeutic approaches, consisting in the administration of 1 to 3 drugs, are adequate to reach adequate blood pressure levels, in some cases more drugs are needed: this condition is called "resistant hypertension". In this context, the administration of a diuretic, such as spironolactone or canrenoate salts, represents a standard practice. Since a reliable discrimination of real cases of resistant hypertension from cases of poor therapeutic adherence is currently difficult to obtain, the adoption of therapeutic drug monitoring has been suggested as a useful tool for this purpose. In this work, the authors developed and validated a simple, cheap and fast dilute-and-shot method with UHPLC-PDA analysis for the quantification of spironolactone and its metabolite canrenone in human urine samples.
94
29
35
Canrenone; Diuretics; Hypertension; Liquid Chromatography; Photodiode array; Spironolactone; Therapeutic drug monitoring; Urine analysis
Avataneo, Valeria; De Nicolò, Amedeo; Rabbia, Franco; Sciandra, Mauro; Tosello, Francesco; Cusato, Jessica; Perlo, Elisa; Fatiguso, Giovanna; Allegra, Sarah; Favata, Fabio; Mulatero, Paolo; Veglio, Franco; Di Perri, Giovanni; D'Avolio, Antonio
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S1056871918306683-main.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 796.3 kB
Formato Adobe PDF
796.3 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1678728
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact