Mapping the activity of neuronal circuits with high resolution in the intact brain is a fundamental step toward understanding brain function. In the last several years, nonlinear microscopy combined with fluorescent activity reporters has become a crucial tool for achieving this goal. In this review article, we will highlight the principles underlying nonlinear microscopy and discuss its application to neuroscience, focusing on recent functional studies in the rodent neocortex in combination with genetically encoded calcium indicators.

Mapping brain circuit function in vivo using two-photon fluorescence microscopy

Bovetti, Serena;
2014-01-01

Abstract

Mapping the activity of neuronal circuits with high resolution in the intact brain is a fundamental step toward understanding brain function. In the last several years, nonlinear microscopy combined with fluorescent activity reporters has become a crucial tool for achieving this goal. In this review article, we will highlight the principles underlying nonlinear microscopy and discuss its application to neuroscience, focusing on recent functional studies in the rodent neocortex in combination with genetically encoded calcium indicators.
2014
77
7
492
501
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0029
Calcium dyes; Central nervous system; Functional imaging; Two-photon excitation; Animals; Brain Mapping; Fluorescent Dyes; Humans; Microscopy, Fluorescence, Multiphoton; Neural Pathways; Anatomy; Histology; Instrumentation; Medical Laboratory Technology
Bovetti, Serena; Moretti, Claudio; Fellin, Tommaso*
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1688629
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