Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides.

Molecular alliance of Lymantria dispar multiple nucleopolyhedrovirus and a short unmodified antisense oligonucleotide of its anti-apoptotic IAP-3 gene: A novel approach for gypsy moth control

Skorokhod, Oleksii A.
Last
2017

Abstract

Baculovirus IAP (inhibitor-of-apoptosis) genes originated by capture of host genes. Unmodified short antisense DNA oligonucleotides (oligoDNAs) from baculovirus IAP genes can down-regulate specific gene expression profiles in both baculovirus-free and baculovirus-infected insects. In this study, gypsy moth (Lymantria dispar) larvae infected with multiple nucleopolyhedrovirus (LdMNPV), and LdMNPV-free larvae, were treated with oligoDNA antisense to the RING (really interesting new gene) domain of the LdMNPV IAP-3 gene. The results with respect to insect mortality, biomass accumulation, histological studies, RT-PCR, and analysis of DNA apoptotic fragmentation suggest that oligoRING induced increased apoptotic processes in both LdMNPV-free and LdMNPV-infected insect cells, but were more pronounced in the latter. These data open up possibilities for promising new routes of insect pest control using antisense phosphodiester DNA oligonucleotides.
18
11
2446
2462
http://www.mdpi.com/1422-0067/18/11/2446/pdf
Baculovirus infection; DNA insecticides; Gypsy moth Lymantria dispar; Insect pest management; Multiple nucleopolyhedrovirus IAP (inhibitor-of-apoptosis) genes; RING (really interesting new gene); Animals; Apoptosis; Genes, Viral; Insect Control; Larva; Moths; Nucleopolyhedrovirus; Transcriptome; Viral Proteins; Oligodeoxyribonucleotides, Antisense; Catalysis; Molecular Biology; Spectroscopy; Computer Science Applications1707 Computer Vision and Pattern Recognition; Physical and Theoretical Chemistry; Organic Chemistry; Inorganic Chemistry
Oberemok, Volodymyr V.; Laikova, Kateryna V.; Zaitsev, Aleksei S.; Shumskykh, Maksym N.; Kasich, Igor N.; Gal’chinsky, Nikita V.; Bekirova, Viktoriya V.; Makarov, Valentin V.; Agranovsky, Alexey A.; Gushchin, Vladimir A.; Zubarev, Ilya V.; Kubyshkin, Anatoly V.; Fomochkina, Iryna I.; Gorlov, Mikhail V.; Skorokhod, Oleksii A.
File in questo prodotto:
File Dimensione Formato  
ijms-18-02446-v2.pdf

Accesso aperto

Descrizione: Articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 2.66 MB
Formato Adobe PDF
2.66 MB Adobe PDF Visualizza/Apri
Oberemok Skorokhod - OligoDNA and virus - ijms 2017.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 2.66 MB
Formato Adobe PDF
2.66 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1688722
Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 7
social impact