During space missions, real-time monitoring of astronauts’ health status is of crucial importance and therefore there is a strong demand for simple analytical devices that astronauts can use to perform clinical chemistry analyses directly onboard. As part of the “IN SITU Bioanalysis” project, we designed a biosensor for analyzing salivary levels of cortisol in astronauts, a marker of chronic stress. The biosensor is based on the Lateral Flow Immunoassay (LFIA) approach coupled with chemiluminescence (CL) detection and comprised a 3D-printed plastic cartridge containing a sealed fluidic element with the LFIA strip, in which the flow of sample and reagents was activated by pressing buttons on the cartridge and sustained by exploiting capillary forces. For measurement, the photon emission was imaged employing a CL reader based on an ultrasensitive cooled charge-coupled device (CCD) camera. The payload was designed to operate in microgravity and to withstand mechanical stress, such as take-off vibrations, and onboard depressurization events, while the microfluidics was developed considering alterations of physical phenomena occurring in microgravity, such as bubble formation, surface wettability and liquid evaporation. The biosensor, which was successfully used by the Italian astronaut Paolo Nespoli during the VITA mission (July-December 2017), demonstrated the feasibility of performing sensitive LFIA analysis of salivary cortisol down to 0.4 ng/mL directly onboard the International Space Station. It could be easily adapted for the analysis of other clinical biomarkers, thus enabling the early diagnosis of diseases and the timely activation of appropriate countermeasures

Chemiluminescence-based biosensor for monitoring astronauts’ health status during space missions: Results from the International Space Station

Di Nardo, Fabio;Anfossi, Laura;Baggiani, Claudio;
2019-01-01

Abstract

During space missions, real-time monitoring of astronauts’ health status is of crucial importance and therefore there is a strong demand for simple analytical devices that astronauts can use to perform clinical chemistry analyses directly onboard. As part of the “IN SITU Bioanalysis” project, we designed a biosensor for analyzing salivary levels of cortisol in astronauts, a marker of chronic stress. The biosensor is based on the Lateral Flow Immunoassay (LFIA) approach coupled with chemiluminescence (CL) detection and comprised a 3D-printed plastic cartridge containing a sealed fluidic element with the LFIA strip, in which the flow of sample and reagents was activated by pressing buttons on the cartridge and sustained by exploiting capillary forces. For measurement, the photon emission was imaged employing a CL reader based on an ultrasensitive cooled charge-coupled device (CCD) camera. The payload was designed to operate in microgravity and to withstand mechanical stress, such as take-off vibrations, and onboard depressurization events, while the microfluidics was developed considering alterations of physical phenomena occurring in microgravity, such as bubble formation, surface wettability and liquid evaporation. The biosensor, which was successfully used by the Italian astronaut Paolo Nespoli during the VITA mission (July-December 2017), demonstrated the feasibility of performing sensitive LFIA analysis of salivary cortisol down to 0.4 ng/mL directly onboard the International Space Station. It could be easily adapted for the analysis of other clinical biomarkers, thus enabling the early diagnosis of diseases and the timely activation of appropriate countermeasures
2019
129
260
268
www.elsevier.com/locate/bios
Chemiluminescence biosensor; Cortisol; International Space Station; Lateral-flow immunoassay; Point-of-care testing; Space medicine; Biotechnology; Biophysics; Biomedical Engineering; Electrochemistry
Zangheri, Martina; Mirasoli, Mara; Guardigli, Massimo; Di Nardo, Fabio; Anfossi, Laura; Baggiani, Claudio; Simoni, Patrizia; Benassai, Mario; Roda, Aldo*
File in questo prodotto:
File Dimensione Formato  
bb19_129_260.pdf

Accesso riservato

Descrizione: articolo principale
Tipo di file: PDF EDITORIALE
Dimensione 2.33 MB
Formato Adobe PDF
2.33 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
bb19_129_260_draft.pdf

Accesso aperto

Descrizione: bozza
Tipo di file: PREPRINT (PRIMA BOZZA)
Dimensione 1.07 MB
Formato Adobe PDF
1.07 MB Adobe PDF Visualizza/Apri
bb19_129_260_si.pdf

Accesso aperto

Descrizione: supporting informations
Tipo di file: MATERIALE NON BIBLIOGRAFICO
Dimensione 146.76 kB
Formato Adobe PDF
146.76 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1691708
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 39
  • ???jsp.display-item.citation.isi??? 34
social impact